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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using ENSA Polyclonal antibody (14518-1-AP)

    WB analysis using 14518-1-AP (same clone as 14518-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 14518-1-AP (ENSA antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14518-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of mouse brain tissue using ENSA Polyclonal antibody (14518-1-AP)

    WB analysis of mouse brain using 14518-1-AP (same clone as 14518-1-PBS)

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 14518-1-AP (ENSA antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14518-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HeLa cells using ENSA Polyclonal antibody (14518-1-AP)

    WB analysis of HeLa using 14518-1-AP (same clone as 14518-1-PBS)

    HeLa cells were subjected to SDS PAGE followed by western blot with 14518-1-AP (ENSA antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14518-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human small intestine tissue using ENSA Polyclonal antibody (14518-1-AP)

    IHC staining of human small intestine using 14518-1-AP (same clone as 14518-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human small intestine tissue slide using 14518-1-AP (ENSA Antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 14518-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon tissue using ENSA Polyclonal antibody (14518-1-AP)

    IHC staining of human colon using 14518-1-AP (same clone as 14518-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 14518-1-AP (ENSA antibody) at dilution of 1:50 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 14518-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon tissue using ENSA Polyclonal antibody (14518-1-AP)

    IHC staining of human colon using 14518-1-AP (same clone as 14518-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 14518-1-AP (ENSA antibody) at dilution of 1:50 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 14518-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using ENSA Polyclonal antibody (14518-1-AP)

    IF Staining of HeLa using 14518-1-AP (same clone as 14518-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using ENSA antibody (14518-1-AP) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002). This data was developed using the same antibody clone with 14518-1-PBS in a different storage buffer formulation.

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ENSA Polyclonal antibody, PBS Only

ENSA Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 14518-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

ARPP-19e, Alpha-endosulfine

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

14518-1-PBS targets ENSA in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ENSA fusion protein Ag5982 相同性解析による交差性が予測される生物種
Full Name endosulfine alpha
Calculated molecular weight 14 kDa
Observed molecular weight 19 kDa
GenBank accession numberBC004461
Gene Symbol ENSA
Gene ID (NCBI) 2029
RRIDAB_10838808
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDO43768
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

Endosulfine alpha (ENSA) belongs to the highly conserved c-AMP-regulated phosphoprotein (ARPP) family and was originally identified as an endogenous ligand for the sulfonylurea receptor, which regulates insulin secretion and glucose metabolism.ENSA not only regulates the cell cycle by interacting with microtubule-associated serine/threonine protein kinase-like (MASTL) enzymes, but also influences tumor growth through its own methylation. Recent studies have shown that ENSA is an important regulator of cholesterol biosynthesis in triple-negative breast cancer (TNBC) and that it triggers tumor growth by promoting cholesterol biosynthesis in TNBC.