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Validation Data Gallery

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  • Western Blot (WB) analysis of HL-60 cells using ENPP4 Polyclonal antibody (17305-1-AP)

    WB analysis of HL-60 using 17305-1-AP (same clone as 17305-1-PBS)

    HL-60 cells were subjected to SDS PAGE followed by western blot with 17305-1-AP (ENPP4 antibody) at dilution of 1:1200 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 17305-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HL-60 cells using ENPP4 Polyclonal antibody (17305-1-AP)

    WB analysis of HL-60 using 17305-1-AP (same clone as 17305-1-PBS)

    HL-60 cells were subjected to SDS PAGE followed by western blot with 17305-1-AP (ENPP4 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 17305-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human brain tissue using ENPP4 Polyclonal antibody (17305-1-AP)

    IHC staining of human brain using 17305-1-AP (same clone as 17305-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human brain using 17305-1-AP (ENPP4 antibody) at dilution of 1:100 (under 10x lens). This data was developed using the same antibody clone with 17305-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human brain tissue using ENPP4 Polyclonal antibody (17305-1-AP)

    IHC staining of human brain using 17305-1-AP (same clone as 17305-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human brain using 17305-1-AP (ENPP4 antibody) at dilution of 1:100 (under 40x lens). This data was developed using the same antibody clone with 17305-1-PBS in a different storage buffer formulation.

ENPP4 Polyclonal antibody, PBS Only

ENPP4 Polyclonal Antibody for WB, IHC, Indirect ELISA
Cat No. 17305-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, Indirect ELISA

Synonyms

Formulation:  PBS Only
PBS, Azide, Glycerol
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

17305-1-PBS targets ENPP4 in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag11192

Product name: Recombinant human ENPP4 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 24-350 aa of BC018054

Sequence: LPPKLLLVSFDGFRADYLKNYEFPHLQNFIKEGVLVEHVKNVFITKTFPNHYSIVTGLYEESHGIVANSMYDAVTKKHFSDSNDKDPFWWNEAVPIWVTNQLQENRSSAAAMWPGTDVPIHDTISSYFMNYNSSVSFEERLNNITMWLNNSNPPVTFATLYWEEPDASGHKYGPEDKENMSRVLKKIDDLIGDLVQRLKMLGLWENLNVIITSDHGMTQCSQDRLINLDSCIDHSYYTLIDLSPVAAILPKINRTEVYNKLKNCSPHMNVYLKEDIPNRFYYQHNDRIQPIILVADEGWTIVLNESSQKLGDHGYDNSLPSMHPFLA

相同性解析による交差性が予測される生物種
Full Name ectonucleotide pyrophosphatase/phosphodiesterase 4 (putative function)
Calculated molecular weight 453 aa, 52 kDa
Observed molecular weight 52-60 kDa
GenBank accession numberBC018054
Gene Symbol ENPP4
Gene ID (NCBI) 22875
RRIDAB_2099780
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9Y6X5
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

ENPP4, also named as KIAA0879 or NPP4, is a 453 amino acid protein, which belongs to the nucleotide pyrophosphatase/phosphodiesterase family. ENPP4 localizes in the cell membrane and hydrolyzes extracellular Ap3A into AMP and ADP, and Ap4A into AMP and ATP. ENPP4 is expressed on the surface of vascular endothelia.

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