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Validation Data Gallery

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  • Western Blot (WB) analysis of Jurkat cells using ENOX1 Polyclonal antibody (23750-1-AP)

    WB analysis of Jurkat using 23750-1-AP

    Jurkat cells were subjected to SDS PAGE followed by western blot with 23750-1-AP (ENOX1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of Raji cells using ENOX1 Polyclonal antibody (23750-1-AP)

    WB analysis of Raji using 23750-1-AP

    Raji cells were subjected to SDS PAGE followed by western blot with 23750-1-AP (ENOX1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of Raji cells using ENOX1 Polyclonal antibody (23750-1-AP)

    WB analysis of Raji using 23750-1-AP

    Raji cells were subjected to SDS PAGE followed by western blot with 23750-1-AP (ENOX1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of MCF-7 cells using ENOX1 Polyclonal antibody (23750-1-AP)

    WB analysis of MCF-7 using 23750-1-AP

    MCF7 cells were subjected to SDS PAGE followed by western blot with 23750-1-AP (ENOX1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of MCF-7 cells using ENOX1 Polyclonal antibody (23750-1-AP)

    WB analysis of MCF-7 using 23750-1-AP

    MCF7 cells were subjected to SDS PAGE followed by western blot with 23750-1-AP (ENOX1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of MCF-7 cells using ENOX1 Polyclonal antibody (23750-1-AP)

    WB analysis of MCF-7 using 23750-1-AP

    MCF7 cells were subjected to SDS PAGE followed by western blot with 23750-1-AP (ENOX1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of Jurkat cells using ENOX1 Polyclonal antibody (23750-1-AP)

    WB analysis of Jurkat using 23750-1-AP

    Jurkat cells were subjected to SDS PAGE followed by western blot with 23750-1-AP (ENOX1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

ENOX1 Polyclonal antibody

ENOX1 Polyclonal Antibody for WB, ELISA
Cat No. 23750-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Synonyms

Ecto-NOX disulfide-thiol exchanger 1, Constitutive Ecto-NOX, cNOX, Cell proliferation-inducing gene 38 protein, cCNOX

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inJurkat cells, MCF-7 cells, Raji cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

23750-1-AP targets ENOX1 in WB, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag20715

Product name: Recombinant human ENOX1 protein

Source: e coli.-derived, PET30a

Tag: 6*His

Domain: 344-643 aa of BC024178

Sequence: TQFEQIVAVFNASTRQKAWDHFSKAQRKNIDIWRKHSEELRNAQSEQLMGIRREEEMEMSDDENCDSPTKKMRVDESALAAQAYALKEENDSLRWQLDAYRNEVELLKQEKEQLFRTEENLTKDQQLQFLQQTMQGMQQQLLTIQEELNNKKSELEQAKEEQSHTQALLKVLQEQLKGTKELVETNGHSHEDSNEINVLTVALVNQDRENNIEKRSQGLKSEKEALLIGIISTFLHVHPFGANIEYLWSYMQQLDSKISANEIEMLLMRLPRMFKQEFTGVGATLEKRWKLCAFEGIKTT

相同性解析による交差性が予測される生物種
Full Name ecto-NOX disulfide-thiol exchanger 1
Calculated molecular weight 73 kDa
Observed molecular weight 73 kDa
GenBank accession numberBC024178
Gene Symbol ENOX1
Gene ID (NCBI) 55068
RRIDAB_2879316
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen Affinity purified
UNIPROT IDQ8TC92
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for ENOX1 antibody 23750-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols