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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using ENOSF1 Polyclonal antibody (31022-1-AP)

    WB analysis using 31022-1-AP (same clone as 31022-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 31022-1-AP (ENOSF1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 31022-1-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of MCF-7 cells using ENOSF1 Polyclonal antibody (31022-1-AP)

    IP experiment of MCF-7 using 31022-1-AP (same clone as 31022-1-PBS)

    IP result of anti-ENOSF1 (IP:31022-1-AP, 4ug; Detection:31022-1-AP 1:2000) with MCF-7 cells lysate 1600 ug. This data was developed using the same antibody clone with 31022-1-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of HeLa cells using ENOSF1 Polyclonal antibody (31022-1-AP)

    FC experiment of HeLa using 31022-1-AP (same clone as 31022-1-PBS)

    1x10^6 HeLa cells were intracellularly stained with 0.25 ug ENOSF1 Polyclonal antibody (31022-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Isotype Control. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer. This data was developed using the same antibody clone with 31022-1-PBS in a different storage buffer formulation.

ENOSF1 Polyclonal antibody, PBS Only

ENOSF1 Polyclonal Antibody for WB, FC (Intra), IP, Indirect ELISA
Cat No. 31022-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, FC (Intra), IP, Indirect ELISA

Synonyms

EC:4.2.1.68, enolase superfamily member 1, L-fuconate dehydratase, rTS, TYMSAS

Formulation:  PBS Only
PBS, Azide, Glycerol
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

31022-1-PBS targets ENOSF1 in WB, FC (Intra), IP, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag34665

Product name: Recombinant human ENOSF1 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-443 aa of BC001285

Sequence: MVRGRISRLSVRDVRFPTSLGGHGADAMHTDPDYSAAYVVIETDAEDGIKGCGITFTLGKGTEVVVCAVNALAHHVLNKDLKDIVGDFRGFYRQLTSDGQLRWIGPEKGVVHLATAAVLNAVWDLWAKQEGKPVWKLLVDMDPRMLVSCIDFRYITDVLTEEDALEILQKGQIGKKEREKQMLAQGYPAYTTSCAWLGYSDDTLKQLCAQALKDGWTRFKVKVGADLQDDMRRCQIIRDMIGPEKTLMMDANQRWDVPEAVEWMSKLAKFKPLWIEEPTSPDDILGHATISKALVPLGIGIATGEQCHNRVIFKQLLQAKALQFLQIDSCRLGSVNENLSVLLMAKKFEIPVCPHAGGVGLCELVQHLIIFDYISVSASLENRVCEYVDHLHEHFKYPVMIQRASYMPPKDPGYSTEMKEESVKKHQYPDGEVWKKLLPAQEN

相同性解析による交差性が予測される生物種
Full Name enolase superfamily member 1
Calculated molecular weight 50 kDa
Observed molecular weight 49 kDa
GenBank accession numberBC001285
Gene Symbol ENOSF1
Gene ID (NCBI) 55556
RRIDAB_3669818
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity Purification
UNIPROT IDQ7L5Y1
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

ENOSF1 (Mitochondrial enolase superfamily member 1) is also named as RTS and TYMSAS. The ENOSF1 gene, responsible for encoding the protein mitochondrial enolase superfamily member 1 (ENOF1), exhibits three distinct isoforms. One of these isoforms has been identified as an l-fuconate dehydratase, actively participating in the catabolism of L-fucose, a sugar integral to the carbohydrate composition of cellular glycoproteins (PMID: 38203276). The ENOSF1 transcript has been shown to act as antisense RNA and inhibit TYMS expression (PMID: 35931051).

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