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Validation Data Gallery

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  • Western Blot (WB) analysis of human skeletal muscle tissue using ENO3 Polyclonal antibody (16421-1-AP)

    WB analysis of human skeletal muscle using 16421-1-AP (same clone as 16421-1-PBS)

    human skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 16421-1-AP (ENO3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16421-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of human brain tissue using ENO3 Polyclonal antibody (16421-1-AP)

    WB analysis of human brain using 16421-1-AP (same clone as 16421-1-PBS)

    human brain tissue were subjected to SDS PAGE followed by western blot with 16421-1-AP (ENO3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16421-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using ENO3 Polyclonal antibody (16421-1-AP)

    IF Staining of HeLa using 16421-1-AP (same clone as 16421-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using ENO3 antibody (16421-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 16421-1-PBS in a different storage buffer formulation.

ENO3 Polyclonal antibody, PBS Only

ENO3 Polyclonal Antibody for WB, IF/ICC, ELISA
Cat No. 16421-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IF/ICC, ELISA

Synonyms

Muscle-specific enolase, MSE, GSD13, EC:4.2.1.11, Beta-enolase

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

16421-1-PBS targets ENO3 in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag9613

Product name: Recombinant human ENO3 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 94-434 aa of BC017249

Sequence: MIELDGTENKSKFGANAILGVSLAVCKAGAAEKGVPLYRHIADLAGNPDLILPVPAFNVINGGSHAGNKLAMQEFMILPVGASSFKEAMRIGAEVYHHLKGVIKAKYGKDATNVGDEGGFAPNILENNEALELLKTAIQAAGYPDKVVIGMDVAASEFYRNGKYDLDFKSPDDPARHITGEKLGELYKSFIKNYPVVSIEDPFDQDDWATWTSFLSGVNIQIVGDDLTVTNPKRIAQAVEKKACNCLLLKVNQIGSVTESIQACKLAQSNGWGVMVSHRSGETEDTFIADLVVGLCTGQIKTGAPCRSERLAKYNQLMRIEEALGDKAIFAGRKFRNPKAK

相同性解析による交差性が予測される生物種
Full Name enolase 3 (beta, muscle)
Calculated molecular weight 434 aa, 47 kDa
Observed molecular weight 47 kDa
GenBank accession numberBC017249
Gene Symbol ENO3
Gene ID (NCBI) 2027
RRIDAB_2099464
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDP13929
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

ENO3, also named as MSE, belongs to the enolase family. It appears to have a function in striated muscle development and regeneration. Defects in ENO3 are the cause of glycogen storage disease type 13 (GSD13).

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