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Validation Data Gallery

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  • Western Blot (WB) analysis of HEK-293 cells using ENO1 Monoclonal antibody (67187-1-Ig)

    WB analysis of HEK-293 using 67187-1-Ig (same clone as 67187-1-PBS)

    WB result of ENO1 antibody (67187-1-Ig; 1:5000; incubated at room temperature for 1.5 hours) with sh-Control and sh-ENO1 transfected HEK-293 cells. This data was developed using the same antibody clone with 67187-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using ENO1 Monoclonal antibody (67187-1-Ig)

    WB analysis using 67187-1-Ig (same clone as 67187-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67187-1-Ig (ENO1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67187-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using ENO1 Monoclonal antibody (67187-1-Ig)

    IF Staining of HeLa using 67187-1-Ig (same clone as 67187-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using ENO1 antibody (67187-1-Ig, Clone: 1D6E8 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1). This data was developed using the same antibody clone with 67187-1-PBS in a different storage buffer formulation.

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  • KD/KO Validated

ENO1 Monoclonal antibody, PBS Only

ENO1 Monoclonal Antibody for WB, IF/ICC, Indirect ELISA
Cat No. 67187-1-PBS
Clone No.1D6E8

Host / Isotype

Mouse / IgM

Reactivity

human, mouse, rat, pig

Applications

WB, IF/ICC, Indirect ELISA

Synonyms

ENO1L1, EC:4.2.1.11, C-myc promoter-binding protein, Alpha-enolase, 2-phospho-D-glycerate hydro-lyase

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

ご購入について

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国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Mouse monoclonal antibodies of IgM isotype can be detected with "anti-mouse IgG (H+L)" secondary antibodies.

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

67187-1-PBS targets ENO1 in WB, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat, pig samples.

Tested Reactivity human, mouse, rat, pig
Host / Isotype Mouse / IgM
Class Monoclonal
Type Antibody
Immunogen ENO1 fusion protein Ag17518 相同性解析による交差性が予測される生物種
Full Name enolase 1, (alpha)
Calculated molecular weight 47 kDa
Observed molecular weight 47 kDa
GenBank accession numberBC015641
Gene Symbol ENO1
Gene ID (NCBI) 2023
ENSEMBL Gene IDENSG00000074800
RRIDAB_2882482
Conjugate Unconjugated
Form Liquid
Purification MethodThiophilic affinity chromatograph
UNIPROT IDP06733
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

ENO1, also named as NNE, ENO1L1, MBPB1, MPB1 and MBP1, belongs to the enolase family. ENO1 is a metabolic enzyme involved in the synthesis of pyruvate. It also acts as a plasminogen receptor and mediates the activation of plasmin and extracellular matrix degradation. In tumor cells, ENO1 is up-regulated and supports the Warburg effect; it is expressed at the cell surface, where it promotes cancer invasion, and is subjected to a specific array of post-translational modifications, namely acetylation, methylation and phosphorylation. ENO1 overexpression and post-translational modifications could be of diagnostic and prognostic value in many cancer types. (PMID: 27814656)