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Validation Data Gallery

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  • Western Blot (WB) analysis of HeLa cells using ENO1 Polyclonal antibody (55237-1-AP)

    WB analysis of HeLa using 55237-1-AP (same clone as 55237-1-PBS)

    HeLa cells were subjected to SDS PAGE followed by western blot with 55237-1-AP (ENO1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 55237-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HEK-293 cells using ENO1 Polyclonal antibody (55237-1-AP)

    WB analysis of HEK-293 using 55237-1-AP (same clone as 55237-1-PBS)

    WB result of ENO1 antibody (55237-1-AP; 1:60000; incubated at room temperature for 1.5 hours) with sh-Control and sh-ENO1 transfected HEK-293 cells. This data was developed using the same antibody clone with 55237-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HeLa cells using ENO1 Polyclonal antibody (55237-1-AP)

    WB analysis of HeLa using 55237-1-AP (same clone as 55237-1-PBS)

    HeLa cells were subjected to SDS PAGE followed by western blot with 55237-1-AP (ENO1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 55237-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HepG2 cells using ENO1 Polyclonal antibody (55237-1-AP)

    WB analysis of HepG2 using 55237-1-AP (same clone as 55237-1-PBS)

    HepG2 cells were subjected to SDS PAGE followed by western blot with 55237-1-AP (ENO1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 55237-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of mouse brain tissue using ENO1 Polyclonal antibody (55237-1-AP)

    WB analysis of mouse brain using 55237-1-AP (same clone as 55237-1-PBS)

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 55237-1-AP (ENO1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 55237-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of mouse brain tissue using ENO1 Polyclonal antibody (55237-1-AP)

    WB analysis of mouse brain using 55237-1-AP (same clone as 55237-1-PBS)

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 55237-1-AP (ENO1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 55237-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HepG2 cells using ENO1 Polyclonal antibody (55237-1-AP)

    WB analysis of HepG2 using 55237-1-AP (same clone as 55237-1-PBS)

    HepG2 cells were subjected to SDS PAGE followed by western blot with 55237-1-AP (ENO1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 55237-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of mouse liver tissue using ENO1 Polyclonal antibody (55237-1-AP)

    WB analysis of mouse liver using 55237-1-AP (same clone as 55237-1-PBS)

    mouse liver tissue were subjected to SDS PAGE followed by western blot with 55237-1-AP (ENO1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 55237-1-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of mouse brain tissue using ENO1 Polyclonal antibody (55237-1-AP)

    IP experiment of mouse brain using 55237-1-AP (same clone as 55237-1-PBS)

    IP result of anti-ENO1 (IP:55237-1-AP, 4ug; Detection:55237-1-AP 1:500) with mouse brain tissue lysate 4000ug. This data was developed using the same antibody clone with 55237-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human brain tissue using ENO1 Polyclonal antibody (55237-1-AP)

    IHC staining of human brain using 55237-1-AP (same clone as 55237-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human brain using 55237-1-AP (ENO1 antibody) at dilution of 1:100 (under 10x lens). This data was developed using the same antibody clone with 55237-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human brain tissue using ENO1 Polyclonal antibody (55237-1-AP)

    IHC staining of human brain using 55237-1-AP (same clone as 55237-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human brain using 55237-1-AP (ENO1 antibody) at dilution of 1:100 (under 40x lens). This data was developed using the same antibody clone with 55237-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human brain tissue using ENO1 Polyclonal antibody (55237-1-AP)

    IHC staining of human brain using 55237-1-AP (same clone as 55237-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human brain using 55237-1-AP (ENO1 antibody) at dilution of 1:100 (under 10x lens). This data was developed using the same antibody clone with 55237-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human skeletal muscle tissue using ENO1 Polyclonal antibody (55237-1-AP)

    IHC staining of human skeletal muscle using 55237-1-AP (same clone as 55237-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human skeletal muscle using 55237-1-AP (ENO1 antibody) at dilution of 1:100 (under 10x lens). This data was developed using the same antibody clone with 55237-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human skeletal muscle tissue using ENO1 Polyclonal antibody (55237-1-AP)

    IHC staining of human skeletal muscle using 55237-1-AP (same clone as 55237-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human skeletal muscle using 55237-1-AP (ENO1 antibody) at dilution of 1:100 (under 40x lens). This data was developed using the same antibody clone with 55237-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human pancreas tissue using ENO1 Polyclonal antibody (55237-1-AP)

    IHC staining of human pancreas using 55237-1-AP (same clone as 55237-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human pancreas using 55237-1-AP (ENO1 antibody) at dilution of 1:100 (under 10x lens). This data was developed using the same antibody clone with 55237-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human pancreas tissue using ENO1 Polyclonal antibody (55237-1-AP)

    IHC staining of human pancreas using 55237-1-AP (same clone as 55237-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human pancreas using 55237-1-AP (ENO1 antibody) at dilution of 1:100 (under 40x lens). This data was developed using the same antibody clone with 55237-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human pancreas tissue using ENO1 Polyclonal antibody (55237-1-AP)

    IHC staining of human pancreas using 55237-1-AP (same clone as 55237-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human pancreas using 55237-1-AP (ENO1 antibody) at dilution of 1:100 (under 10x lens). This data was developed using the same antibody clone with 55237-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human pancreas tissue using ENO1 Polyclonal antibody (55237-1-AP)

    IHC staining of human pancreas using 55237-1-AP (same clone as 55237-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human pancreas using 55237-1-AP (ENO1 antibody) at dilution of 1:100 (under 40x lens). This data was developed using the same antibody clone with 55237-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human brain tissue using ENO1 Polyclonal antibody (55237-1-AP)

    IHC staining of human brain using 55237-1-AP (same clone as 55237-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human brain using 55237-1-AP (ENO1 antibody) at dilution of 1:100 (under 40x lens). This data was developed using the same antibody clone with 55237-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using ENO1 Polyclonal antibody (55237-1-AP)

    IF Staining of HepG2 using 55237-1-AP (same clone as 55237-1-PBS)

    Immunofluorescent analysis of HepG2 cells using 55237-1-AP (ENO1 antibody) at dilution of 1:50 and Rhodamine-Goat anti-Rabbit IgG. This data was developed using the same antibody clone with 55237-1-PBS in a different storage buffer formulation.

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  • Featured Product
  • KD/KO Validated

ENO1 Polyclonal antibody, PBS Only

ENO1 Polyclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA
Cat No. 55237-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, IP, Indirect ELISA

Synonyms

Enolase 1, ENO1L1, EC:4.2.1.11, C-myc promoter-binding protein, Alpha-enolase

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

55237-1-PBS targets ENO1 in WB, IHC, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide 相同性解析による交差性が予測される生物種
Full Name enolase 1, (alpha)
Calculated molecular weight 47 kDa
Observed molecular weight 47 kDa
GenBank accession numberNM_001428
Gene Symbol ENO1
Gene ID (NCBI) 2023
ENSEMBL Gene IDENSG00000074800
RRIDAB_10837362
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP06733
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

ENO1, also named as NNE, ENO1L1, MBPB1, MPB1 and MBP1, belongs to the enolase family. ENO1 is a metabolic enzyme involved in the synthesis of pyruvate. It also acts as a plasminogen receptor and mediates the activation of plasmin and extracellular matrix degradation. In tumor cells, ENO1 is up-regulated and supports the Warburg effect; it is expressed at the cell surface, where it promotes cancer invasion, and is subjected to a specific array of post-translational modifications, namely acetylation, methylation and phosphorylation. ENO1 overexpression and post-translational modifications could be of diagnostic and prognostic value in many cancer types. (PMID: 27814656). This antibody is specific to ENO1 and has no cross reaction with ENO2 and ENO3.