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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using EML4 Polyclonal antibody (22709-1-AP)

    WB analysis using 22709-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 22709-1-AP (EML4 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of A549 cells using EML4 Polyclonal antibody (22709-1-AP)

    IP experiment of A549 using 22709-1-AP

    IP result of anti-EML4 (IP:22709-1-AP, 4ug; Detection:22709-1-AP 1:2000) with A549 cells lysate 400ug.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using EML4 Polyclonal antibody (22709-1-AP)

    IHC staining of human breast cancer using 22709-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 22709-1-AP (EML4 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using EML4 Polyclonal antibody (22709-1-AP)

    IHC staining of human breast cancer using 22709-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 22709-1-AP (EML4 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human pancreas cancer tissue using EML4 Polyclonal antibody (22709-1-AP)

    IHC staining of human pancreas cancer using 22709-1-AP

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 22709-1-AP (EML4 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human placenta tissue using EML4 Polyclonal antibody (22709-1-AP)

    IHC staining of human placenta using 22709-1-AP

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 22709-1-AP (EML4 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse liver tissue using EML4 Polyclonal antibody (22709-1-AP)

    IHC staining of mouse liver using 22709-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 22709-1-AP (EML4 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of rat liver tissue using EML4 Polyclonal antibody (22709-1-AP)

    IHC staining of rat liver using 22709-1-AP

    Immunohistochemical analysis of paraffin-embedded rat liver tissue slide using 22709-1-AP (EML4 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of rat small intestine tissue using EML4 Polyclonal antibody (22709-1-AP)

    IHC staining of rat small intestine using 22709-1-AP

    Immunohistochemical analysis of paraffin-embedded rat small intestine tissue slide using 22709-1-AP (EML4 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of rat pancreas tissue using EML4 Polyclonal antibody (22709-1-AP)

    IHC staining of rat pancreas using 22709-1-AP

    Immunohistochemical analysis of paraffin-embedded rat pancreas tissue slide using 22709-1-AP (EML4 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using EML4 Polyclonal antibody (22709-1-AP)

    IHC staining of human lung cancer using 22709-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 22709-1-AP (EML4 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using EML4 Polyclonal antibody (22709-1-AP)

    IHC staining of human lung cancer using 22709-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 22709-1-AP (EML4 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human prostate cancer tissue using EML4 Polyclonal antibody (22709-1-AP)

    IHC staining of human prostate cancer using 22709-1-AP

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 22709-1-AP (EML4 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human prostate cancer tissue using EML4 Polyclonal antibody (22709-1-AP)

    IHC staining of human prostate cancer using 22709-1-AP

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 22709-1-AP (EML4 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of A549 cells using EML4 Polyclonal antibody (22709-1-AP)

    IF Staining of A549 using 22709-1-AP

    Immunofluorescent analysis of (-20°C Methanol) fixed A549 cells using EML4 antibody (22709-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2).

  • Immunofluorescence (IF) / fluorescent staining of A549 cells using EML4 Polyclonal antibody (22709-1-AP)

    IF Staining of A549 using 22709-1-AP

    Immunofluorescent analysis of (-20℃ Ethanol) fixed A549 cells using 22709-1-AP (EML4 antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

EML4 Polyclonal antibody

EML4 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA
Cat No. 22709-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, ELISA

Synonyms

C2orf2, Echinoderm microtubule-associated protein-like 4, EMAPL4, Restrictedly overexpressed proliferation-associated protein

Formulation:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inA431 cells, A549 cells, Calu-1 cells, HeLa cells
Positive IP detected inA549 cells
Positive IHC detected inhuman breast cancer tissue, human lung cancer tissue, human prostate cancer tissue, human pancreas cancer tissue, human placenta tissue, mouse liver tissue, rat liver tissue, rat small intestine tissue, rat pancreas tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inA549 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:8000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

22709-1-AP targets EML4 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag18463

Product name: Recombinant human EML4 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 6-663 aa of BC140845

Sequence: GSLDDSISAASTSDVQDRLSALESRVQQQEDEITVLKAALADVLRRLAISEDHVASVKKSVSSKGQPSPRAVIPMSCITNGSGANRKPSHTSAVSIAGKETLSSAAKSGTEKKKEKPQGQREKKEESHSNDQSPQIRASPSPQPSSQPLQIHRQTPESKNATPTKSIKRPSPAEKSHNSWENSDDSRNKLSKIPSTPKLIPKVTKTADKHKDVIINQEGEYIKMFMRGRPITMFIPSDVDNYDDIRTELPPEKLKLEWAYGYRGKDCRANVYLLPTGEIVYFIASVVVLFNYEERTQRHYLGHTDCVKCLAIHPDKIRIATGQIAGVDKDGRPLQPHVRVWDSVTLSTLQIIGLGTFERGVGCLDFSKADSGVHLCVIDDSNEHMLTVWDWQKKAKGAEIKTTNEVVLAVEFHPTDANTIITCGKSHIFFWTWSGNSLTRKQGIFGKYEKPKFVQCLAFLGNGDVLTGDSGGVMLIWSKTTVEPTPGKGPKGVYQISKQIKAHDGSVFTLCQMRNGMLLTGGGKDRKIILWDHDLNPEREIEVPDQYGTIRAVAEGKADQFLVGTSRNFILRGTFNDGFQIEVQGHTDELWGLATHPFKDLLLTCAQDRQVCLWNSMEHRLEWTRLVDEPGHCADFHPSGTVVAIGTHSGRWFVLDAE

相同性解析による交差性が予測される生物種
Full Name echinoderm microtubule associated protein like 4
Calculated molecular weight 981 aa, 109 kDa
Observed molecular weight 120 kDa
GenBank accession numberBC140845
Gene Symbol EML4
Gene ID (NCBI) 27436
RRIDAB_2879153
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen Affinity purified
UNIPROT IDQ9HC35
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
IF protocol for EML4 antibody 22709-1-APDownload protocol
IHC protocol for EML4 antibody 22709-1-APDownload protocol
IP protocol for EML4 antibody 22709-1-APDownload protocol
WB protocol for EML4 antibody 22709-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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