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Validation Data Gallery

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  • Western Blot (WB) analysis of A375 cells using EME1 Polyclonal antibody (12975-1-AP)

    WB analysis of A375 using 12975-1-AP (same clone as 12975-1-PBS)

    A375 cells were subjected to SDS PAGE followed by western blot with 12975-1-AP (EME1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12975-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of human liver tissue using EME1 Polyclonal antibody (12975-1-AP)

    WB analysis of human liver using 12975-1-AP (same clone as 12975-1-PBS)

    human liver tissue were subjected to SDS PAGE followed by western blot with 12975-1-AP (EME1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12975-1-PBS in a different storage buffer formulation.

EME1 Polyclonal antibody, PBS Only

EME1 Polyclonal Antibody for WB, Indirect ELISA
Cat No. 12975-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, Indirect ELISA

Synonyms

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

12975-1-PBS targets EME1 in WB, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag3580

Product name: Recombinant human EME1 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 234-584 aa of BC016470

Sequence: AALVTRMKAQRPEECLKHIIVVLDPVLLQMEGGGQLLGALQTMECRCVIEAQAVPCSVTWRRRAGPSEDREDWVEEPTVLVLLRAEAFVSMIDNGKQGSLDSTMKGKETLQGFVTDITAKTAGKALSLVIVDQEKCFSLELLFFDFLPCTSAQNPPRRGKQGANKQTKKQQQRQPEASIGSMVSRVDAEEALVDLQLHTEAQAQIVQSWKELADFTCAFTKAVAEAPFKKLRDETTFSFCLESDWAGGVKVDLAGRGLALVWRRQIQQLNRVSLEMASAVVNAYPSPQLLVQAYQQCFSDKERQNLLADIQVRRGEGVTSTSRRIGPELSRRIYLQMTTLQPHLSLDSAD

相同性解析による交差性が予測される生物種
Full Name essential meiotic endonuclease 1 homolog 1 (S. pombe)
Calculated molecular weight 583 aa, 65 kDa
Observed molecular weight 65 kDa
GenBank accession numberBC016470
Gene Symbol EME1
Gene ID (NCBI) 146956
RRIDAB_2231333
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ96AY2
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.
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