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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

EMD Recombinant antibody

EMD Recombinant Antibody for IF, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human

Applications

IF, ELISA

Conjugate

Unconjugated

CloneNo.

230089B1

Cat no : 82888-1-RR

Synonyms

EDMD, EMD, emerin, LEMD5, STA

Validation Data Gallery

Applications:
  • IF Staining of HeLa using 82888-1-RR

    IF Staining of HeLa using 82888-1-RR

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using EMD antibody (82888-1-RR, Clone: 230089B1 ) at dilution of 1:1000 and Multi-rAb CoraLite ® Plus 594-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (RGAM004).(CL488-Phalloidin, green).

Tested Applications

Positive IF detected inHeLa cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)IF : 1:500-1:2000
Sample-dependent, check data in validation data gallery

Product Information

82888-1-RR targets EMD in IF, ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen EMD fusion protein Ag0236 相同性解析による交差性が予測される生物種
Full Name emerin
Calculated molecular weight 34 kDa
GenBank accession numberBC000738
Gene symbol EMD
Gene ID (NCBI) 2010
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Emerin (Emery-Dreifuss muscular dystrophy) (EMD or EDMD), a serine-rich nuclear membrane protein, is a member of the nuclear lamina-associated protein family. EMD may mediate membrane anchorage to the cytoskeleton by stabilizing and promoting the formation of a nuclear actin cortical network. Defects in EMD gene are the cause of Emery-Dreifuss muscular dystrophy type 1 (EDMD1), a degenerative myopathy characterized by weakness and atrophy of muscle without involvement of the nervous system, early contractures of the elbows Achilles tendons and spine, and cardiomyopathy associated with cardiac conduction defects. EMD inhibits beta-catenin activity by preventing its accumulation in the nucleus and is involved in HIV-1 infection.

Protocols

Product Specific Protocols
IF protocol for EMD antibody 82888-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols