Validation Data Gallery
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Tested Applications
| Positive WB detected in | HUVEC cells, bEnd.3 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:6000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 1 publications below |
| IF | See 3 publications below |
Product Information
67854-1-Ig targets EMCN in WB, IF, ELISA applications and shows reactivity with Human, Mouse samples.
| Tested Reactivity | Human, Mouse |
| Cited Reactivity | human, mouse, rat |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag31146 Product name: Recombinant human EMCN protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 1-178 aa of BC017781 Sequence: MELLQVTILFLLPSICSSNSTGVLEAANNSLVVTTTKPSITTPNTESLQKNVVTPTTGTTPKGTITNELLKMSLMSTATFLTSKDEGLKATTTDVRKNDSIISNVTVTSVTLPNAVSTLQSSKPKTETQSSIKTTEIPGTPENGNDQPQSDKESVKLLTVKTISHESGEHSAQGKTKN 相同性解析による交差性が予測される生物種 |
| Full Name | endomucin |
| Calculated molecular weight | 19 kDa, 27 kDa |
| Observed molecular weight | 120 kDa |
| GenBank accession number | BC017781 |
| Gene Symbol | EMCN |
| Gene ID (NCBI) | 51705 |
| RRID | AB_2918612 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Protein G purification |
| UNIPROT ID | Q9ULC0 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Aliquoting is unnecessary for -20oC storage. |
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for EMCN antibody 67854-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
J Cell Mol Med Human serum-derived exosomes modulate macrophage inflammation to promote VCAM1-mediated angiogenesis and bone regeneration | ||
Cell Signal Forkhead box C1 promotes the pathology of osteoarthritis in subchondral bone osteoblasts via the Piezo1/YAP axis | ||
Commun Biol Piezo1 promotes vibration-induced vascular smooth muscle injury by regulating the NF-κB/p65 axis | ||
Mater Today Bio Human urine-derived stem cell exosomes delivered via injectable GelMA templated hydrogel accelerate bone regeneration |
