Validation Data Gallery
Tested Applications
| Positive WB detected in | HEK-293T cells, Jurkat cells, MCF-7 cells, LNCaP cells |
| Positive IF/ICC detected in | Jurkat cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
86946-1-RR targets EMB in WB, IF/ICC, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Recombinant |
| Type | Antibody |
| Immunogen |
CatNo: Eg6407 Product name: Recombinant human EMB protein Source: mammalian cells-derived, V37 Tag: C-rFc Domain: 33-260 aa of NM_198449.3 Sequence: DGSAPDSPFTSPPLREEIMANNFSLESHNISLTEHSSMPVEKNITLERPSNVNLTCQFTTSGDLNAVNVTWKKDGEQLENNYLVSATGSTLYTQYRFTIINSKQMGSYSCFFREEKEQRGTFNFKVPELHGKNKPLISYVGDSTVLTCKCQNCFPLNWTWYSSNGSVKVPVGVQMNKYVINGTYANETKLKITQLLEEDGESYWCRALFQLGESEEHIELVVLSYLVP 相同性解析による交差性が予測される生物種 |
| Full Name | embigin homolog (mouse) |
| Calculated molecular weight | 37 kDa |
| Observed molecular weight | 60-75 kDa |
| GenBank accession number | NM_198449.3 |
| Gene Symbol | EMB |
| Gene ID (NCBI) | 133418 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | Q6PCB8 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
EMB, also named as Embigin, is a 327 amino acid protein, which contains two Ig-like V-type (immunoglobulin-like) domains. EMB as a membrane protein may unction as a regulator of cell/ECM interactions during development and in the homeostasis of normal adult tissues. EMB is a transmembrane glycoprotein with a protein component of 34 kDa, but western blot analysis of embigin in mouse soleus muscles detects a glycosylated full-length protein with an molecular mass of 70 kDa.(PMID: 19164284)
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for EMB antibody 86946-1-RR | Download protocol |
| WB protocol for EMB antibody 86946-1-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |