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Validation Data Gallery

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  • Western Blot (WB) analysis of A549 cells using EMAP II Polyclonal antibody (11091-1-AP)

    WB analysis of A549 using 11091-1-AP (same clone as 11091-1-PBS)

    WB result of EMAP II antibody (11091-1-AP; 1:6000; incubated at room temperature for 1.5 hours) with sh-Control and sh-EMAP II transfected A549 cells. This data was developed using the same antibody clone with 11091-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using EMAP II Polyclonal antibody (11091-1-AP)

    WB analysis using 11091-1-AP (same clone as 11091-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 11091-1-AP (EMAP II antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11091-1-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of SGC-7901 cells using EMAP II Polyclonal antibody (11091-1-AP)

    IP experiment of SGC-7901 using 11091-1-AP (same clone as 11091-1-PBS)

    IP result of anti-EMAP II (IP:11091-1-AP, 4ug; Detection:11091-1-AP 1:500) with SGC-7901 cells lysate 2200 ug. This data was developed using the same antibody clone with 11091-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human cervical cancer tissue using EMAP II Polyclonal antibody (11091-1-AP)

    IHC staining of human cervical cancer using 11091-1-AP (same clone as 11091-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 11091-1-AP (EMAP II antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11091-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human cervical cancer tissue using EMAP II Polyclonal antibody (11091-1-AP)

    IHC staining of human cervical cancer using 11091-1-AP (same clone as 11091-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 11091-1-AP (EMAP II antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11091-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using EMAP II Polyclonal antibody (11091-1-AP)

    IF Staining of HeLa using 11091-1-AP (same clone as 11091-1-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using EMAP II antibody (11091-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 11091-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO Validated

EMAP II Polyclonal antibody, PBS Only

EMAP II Polyclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA
Cat No. 11091-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, IP, Indirect ELISA

Synonyms

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

11091-1-PBS targets EMAP II in WB, IHC, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag1560

Product name: Recombinant human SCYE1 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-312 aa of BC014051

Sequence: MANNDAVLKRLEQKGAEADQIIEYLKQQVSLLKEKAILQATLREEKKLRVENAKLKKEIEELKQELIQAEIQNGVKQIPFPSGTPLHANSMVSENVIQSTAVTTVSSGTKEQIKGGTGDEKKAKEKIEKKGEKKEKKQQSIAGSADSKPIDVSRLDLRIGCIITARKHPDADSLYVEEVDVGEIAPRTVVSGLVNHVPLEQMQNRMVILLCNLKPAKMRGVLSQAMVMCASSPEKIEILAPPNGSVPGDRITFDAFPGEPDKELNPKKKIWEQIQPDLHTNDECVATYKGVPFEVKGKGVCRAQTMSNSGIK

相同性解析による交差性が予測される生物種
Full Name small inducible cytokine subfamily E, member 1 (endothelial monocyte-activating)
Calculated molecular weight 34 kDa
Observed molecular weight 34 kDa and 68 kDa
GenBank accession numberBC014051
Gene Symbol EMAP II
Gene ID (NCBI) 9255
RRIDAB_2182590
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ12904
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

SCYE1(small inducible cytokine subfamily E, member 1) is also named as EMAP2, AIMP1. The human cDNA encodes a 312-amino acid polypeptide that the predicted 34-kDa precursor can be cleaved to produce an active 20-kDa product(PMID:7929199). SCYE1 is secreted as a result of various stimuli, including TNF-α, heat shock, and hypoxia, and that it acts as a multifunctional cytokine on both endothelial and immune cells(PMID:18292511). Defects in AIMP1 are the cause of leukodystrophy hypomyelinating type 3 (HLD3). It has 2 isoforms produced by alternative splicing and the protein can exsit as a dimer(PMID:11306575).

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