Validation Data Gallery
Tested Applications
| Positive IHC detected in | human stomach cancer tissue, mouse liver tissue, human colon cancer tissue, mouse kidney tissue, rat kidney tissue, rat liver tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Immunohistochemistry (IHC) | IHC : 1:250-1:1000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
13793-1-AP targets ELP2 in IHC, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag4581 Product name: Recombinant human ELP2 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 406-756 aa of BC032553 Sequence: AITGQSLNHVLCNQDSDLPEGATVPALGLSNKAVFQGDIASQPSDEEELLTSTGFEYQQVAFQPSILTEPPTEDHLLQNTLWPEVQKLYGHGYEIFCVTCNSSKTLLASACKAAKKEHAAIILWNTTSWKQVQNLVFHSLTVTQMAFSPNEKFLLAVSRDRTWSLWKKQDTISPEFEPVFSLFAFTNKITSVHSRIIWSCDWSPDSKYFFTGSRDKKVVVWGECDSTDDCIEHNIGPCSSVLDVGGAVTAVSVCPVLHPSQRYVVAVGLECGKICLYTWKKTDQVPEINDWTHCVETSQSQSHTLAIRKLCWKNCSGKTEQKEAEGAEWLHFASCGEDHTVKIHRVNKCAL 相同性解析による交差性が予測される生物種 |
| Full Name | elongation protein 2 homolog (S. cerevisiae) |
| Calculated molecular weight | 826 aa, 93 kDa |
| GenBank accession number | BC032553 |
| Gene Symbol | ELP2 |
| Gene ID (NCBI) | 55250 |
| RRID | AB_3085423 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q6IA86 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
ELP2 is a component of the elongator complex which is required for multiple tRNA modifications. The elongator complex catalyzes the formation of carboxymethyluridine in the wobble base at position 34 in tRNAs.
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for ELP2 antibody 13793-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |







