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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using ELOVL4 Polyclonal antibody (55023-1-AP)

    WB analysis using 55023-1-AP (same clone as 55023-1-PBS)

    Neuro-2a cells were subjected to SDS PAGE followed by western blot with 55023-1-AP (ELOVL4 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 55023-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of Y79 cells using ELOVL4 Polyclonal antibody (55023-1-AP)

    WB analysis of Y79 using 55023-1-AP (same clone as 55023-1-PBS)

    Y79 cells were subjected to SDS PAGE followed by western blot with 55023-1-AP (ELOVL4 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 55023-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of A431 cells using ELOVL4 Polyclonal antibody (55023-1-AP)

    WB analysis of A431 using 55023-1-AP (same clone as 55023-1-PBS)

    A431 cells were subjected to SDS PAGE followed by western blot with 55023-1-AP (ELOVL4 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 55023-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of mouse brain tissue using ELOVL4 Polyclonal antibody (55023-1-AP)

    WB analysis of mouse brain using 55023-1-AP (same clone as 55023-1-PBS)

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 55023-1-AP (ELOVL4 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 55023-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human small intestine tissue using ELOVL4 Polyclonal antibody (55023-1-AP)

    IHC staining of human small intestine using 55023-1-AP (same clone as 55023-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human small intestine using 55023-1-AP (ELOVL4 antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 55023-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human small intestine tissue using ELOVL4 Polyclonal antibody (55023-1-AP)

    IHC staining of human small intestine using 55023-1-AP (same clone as 55023-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human small intestine using 55023-1-AP (ELOVL4 antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 55023-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using ELOVL4 Polyclonal antibody (55023-1-AP)

    IHC staining of mouse brain using 55023-1-AP (same clone as 55023-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 55023-1-AP (ELOVL4 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 55023-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human gliomas tissue using ELOVL4 Polyclonal antibody (55023-1-AP)

    IHC staining of human gliomas using 55023-1-AP (same clone as 55023-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 55023-1-AP (ELOVL4 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 55023-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human gliomas tissue using ELOVL4 Polyclonal antibody (55023-1-AP)

    IHC staining of human gliomas using 55023-1-AP (same clone as 55023-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 55023-1-AP (ELOVL4 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 55023-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of A431 cells using ELOVL4 Polyclonal antibody (55023-1-AP)

    IF Staining of A431 using 55023-1-AP (same clone as 55023-1-PBS)

    Immunofluorescent analysis of A431 cells using 55023-1-AP (ELOVL4 antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 55023-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO Validated

ELOVL4 Polyclonal antibody, PBS Only

ELOVL4 Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 55023-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

STGD2, ELOVL fatty acid elongase 4, ELOVL FA elongase 4, EC:2.3.1.199, ADMD

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

55023-1-PBS targets ELOVL4 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

Peptide

相同性解析による交差性が予測される生物種
Full Name elongation of very long chain fatty acids (FEN1/Elo2, SUR4/Elo3, yeast)-like 4
Calculated molecular weight 37 kDa
Observed molecular weight37-40 kDa
GenBank accession numberNM_022726
Gene Symbol ELOVL4
Gene ID (NCBI) 6785
RRIDAB_10859792
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9GZR5
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

ELOVL4, also named as ADMD, STGD2 and STGD3, belongs to the ELO family. It is a component of the fatty acid elongation system. Elovl4 is involved in the biosynthesis of very long chain fatty acids. It seems to represent a photoreceptor-specific component of the fatty acid elongation system residing on the endoplasmic reticulum. ELOVL4 may be implicated in docosahexaenoic acid (DHA) biosynthesis, which requires dietary consumption of the essential alpha-linolenic acid and a subsequent series of three elongation steps. Defects in ELOVL4 are the cause of Stargardt disease type 3 (STGD3). The antibody is specific to ELOVL4.

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