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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using ELOVL3 Polyclonal antibody (32474-1-AP)

    WB analysis using 32474-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 32474-1-AP (ELOVL3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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ELOVL3 Polyclonal antibody

ELOVL3 Polyclonal Antibody for WB, ELISA
Cat No. 32474-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, ELISA

Synonyms

3-keto acyl-CoA synthase ELOVL3, Cold-inducible glycoprotein of 30 kDa, EC:2.3.1.199, Elongation of very long chain fatty acids protein 3, ELOVL FA elongase 3

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inhuman testis tissue, mouse adipose tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

32474-1-AP targets ELOVL3 in WB, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ELOVL3 fusion protein Ag24318 相同性解析による交差性が予測される生物種
Full Name elongation of very long chain fatty acids (FEN1/Elo2, SUR4/Elo3, yeast)-like 3
Calculated molecular weight 32 kDa
Observed molecular weight32 kDa
GenBank accession numberBC034344
Gene Symbol ELOVL3
Gene ID (NCBI) 83401
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity Purification
UNIPROT IDQ9HB03
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

ELOVL3 (elongation of very long-chain fatty acids 3) is a member of the ELOVL family of enzymes that synthesize very long chain fatty acids (VLCFAs). ELOVL3 is expressed primarily in brown and white adipose tissue, skin sebaceous glands, and the liver, synthesizes C20-C24 saturated and monounsaturated fatty acids (PMID: 37847682). ELOVL3 is involved in the regulation of the progression of adipogenesis through activation of peroxisome proliferator-activated receptor (PPAR)γ in mouse adipocytic 3T3-L1 cells (PMID: 22436697, 16326704).

Protocols

Product Specific Protocols
WB protocol for ELOVL3 antibody 32474-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols