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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using ELMO1 Polyclonal antibody (26974-1-AP)

    WB analysis using 26974-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 26974-1-AP (ELMO1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human placenta tissue using ELMO1 Polyclonal antibody (26974-1-AP)

    IHC staining of human placenta using 26974-1-AP

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 26974-1-AP (ELMO1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse spleen tissue using ELMO1 Polyclonal antibody (26974-1-AP)

    IHC staining of mouse spleen using 26974-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 26974-1-AP (ELMO1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse spleen tissue using ELMO1 Polyclonal antibody (26974-1-AP)

    IHC staining of mouse spleen using 26974-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 26974-1-AP (ELMO1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of Jurkat cells using ELMO1 Polyclonal antibody (26974-1-AP)

    IF Staining of Jurkat using 26974-1-AP

    Immunofluorescent analysis of (4% PFA) fixed Jurkat cells using ELMO1 antibody (26974-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

  • Immunofluorescence (IF) / fluorescent staining of K-562 cells using ELMO1 Polyclonal antibody (26974-1-AP)

    IF Staining of K-562 using 26974-1-AP

    Immunofluorescent analysis of (4% PFA) fixed K-562 cells using ELMO1 antibody (26974-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

［PR］まずは試したいあなたに。トライアルサイズも特別価格 >>『PRIME BUY抗体特割キャンペーン（4/21～）』

ELMO1 Polyclonal antibody

ELMO1 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 26974-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

ELMO 1, engulfment and cell motility 1

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inJurkat cells, mouse brain tissue, rat brain tissue
Positive IHC detected inmouse spleen tissue, human placenta tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inK-562 cells, Jurkat cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

26974-1-AP targets ELMO1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ELMO1 fusion protein Ag25635 相同性解析による交差性が予測される生物種
Full Name engulfment and cell motility 1
Observed molecular weight80~84 kDa
GenBank accession numberBC114341
Gene Symbol ELMO1
Gene ID (NCBI) 9844
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ92556
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

ELMO1 (Engulfment and cell motility protein 1) is a cytoplasmic adapter protein that physically associates with members of the Dock-A family of Rac-GEFs, of which Dock1 and Dock2 are the best characterized (PMID: 24821968). ELMO1 is expressed in platelets and negatively regulates integrin-mediated platelet spreading. Upregulated ELMO1 expression is associated with a poor prognosis in hepatocellular carcinoma (HCC), as well as increased cell growth, invasion, migration, angiogenesis and EMT in vitro and in vivo (PMID: 31324602).

Protocols

Product Specific Protocols
WB protocol for ELMO1 antibody 26974-1-APDownload protocol
IHC protocol for ELMO1 antibody 26974-1-APDownload protocol
IF protocol for ELMO1 antibody 26974-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols