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Validation Data Gallery

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  • Western Blot (WB) analysis of HeLa cells using ELL Polyclonal antibody (51044-1-AP)

    WB analysis of HeLa using 51044-1-AP (same clone as 51044-1-PBS)

    HeLa cells were subjected to SDS PAGE followed by western blot with 51044-1-AP (ELL antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 51044-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HeLa cells using ELL Polyclonal antibody (51044-1-AP)

    WB analysis of HeLa using 51044-1-AP (same clone as 51044-1-PBS)

    HeLa cells were subjected to SDS PAGE followed by western blot with 51044-1-AP (ELL antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 51044-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of mouse testis tissue using ELL Polyclonal antibody (51044-1-AP)

    WB analysis of mouse testis using 51044-1-AP (same clone as 51044-1-PBS)

    mouse testis tissue were subjected to SDS PAGE followed by western blot with 51044-1-AP (ELL antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 51044-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HL-60 cells using ELL Polyclonal antibody (51044-1-AP)

    WB analysis of HL-60 using 51044-1-AP (same clone as 51044-1-PBS)

    HL-60 cells were subjected to SDS PAGE followed by western blot with 51044-1-AP (ELL antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 51044-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HEK-293 cells using ELL Polyclonal antibody (51044-1-AP)

    WB analysis of HEK-293 using 51044-1-AP (same clone as 51044-1-PBS)

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 51044-1-AP (ELL antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 51044-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human ovary tumor tissue using ELL Polyclonal antibody (51044-1-AP)

    IHC staining of human ovary tumor using 51044-1-AP (same clone as 51044-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human ovary tumor using 51044-1-AP (ELL antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 51044-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO Validated

ELL Polyclonal antibody, PBS Only

ELL Polyclonal Antibody for WB, IHC, Indirect ELISA
Cat No. 51044-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, Indirect ELISA

Synonyms

C19orf17, Eleven-nineteen lysine-rich leukemia protein, ELL1, MEN, PPP1R68

Formulation:  PBS Only
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

51044-1-PBS targets ELL in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag0617

Product name: Recombinant human ELL protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-500 aa of BC049195

Sequence: MAALKEDRSYGLSCGRVSDGSKVSVFHVKLTDSALRAFESYRARQDSVSLRPSIRFQGSQGHISIPQPDCPAEARTFSFYLSNIGRDNPQGSFDCIQQYVSSHGEVHLDCLGSIQDKITVCATDDSYQKARQSMAQAEEETRSRSAIVIKAGGRYLGKKVQFRKPAPGATDAVPSRKRATPINLASAIRKSGASAVSGGSGVSQRPFRDRVLHLLALRPYRKAELLLRLQKDGLTQADKDALDGLLQQVANMSAKDGTCTLQDCMYKDVQKDWPGYSEGDQQLLKRVLVRKLCQPQSTGSLLGDPAASSPPGERGRSASPPQKRLQPPDFIDPLANKKPRISHFTQRAQPAVNGKLGVPNGREALLPTPGPPASTDTLSSSTHLPPRLEPPRAHDPLADVSNDLGHSGRDCEHGEAAAPAPTVRLGLPLLTDCAQPSRPHGSPSRSKPKKKSKKHKDKERAAEDKPRAQLPDCAPATHATPGAPADTPGLNGTCSVSSVP

相同性解析による交差性が予測される生物種
Full Name elongation factor RNA polymerase II
Calculated molecular weight 500 aa, 68 kDa
Observed molecular weight 68-80 kDa
GenBank accession numberBC049195
Gene Symbol ELL
Gene ID (NCBI) 8178
RRIDAB_2099137
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDP55199
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

ELL is an elongation factor that inrecase the catalytic rate of RNA polymerase II transcription via suppressing transient pausing by the polymerase at multiple sites along the DNA. Also it's the second elongation factor to be involved in oncogenesis which is a transcription factor regulated by the product of the von Hippel-Lindau tumor suppressor. There are two transcirpts variants in mRNA level, one is 4.4kb transcript and the other is 2.8kb transcript.

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