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Validation Data Gallery

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  • Western Blot (WB) analysis of Jurkat cells using ELF4 Polyclonal antibody (16020-1-AP)

    WB analysis of Jurkat using 16020-1-AP

    Jurkat cells were subjected to SDS PAGE followed by western blot with 16020-1-AP (ELF4 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse spleen tissue using ELF4 Polyclonal antibody (16020-1-AP)

    WB analysis of mouse spleen using 16020-1-AP

    mouse spleen tissue were subjected to SDS PAGE followed by western blot with 16020-1-AP (ELF4 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of PC-3 cells using ELF4 Polyclonal antibody (16020-1-AP)

    WB analysis of PC-3 using 16020-1-AP

    PC-3 cells were subjected to SDS PAGE followed by western blot with 16020-1-AP (ELF4 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using ELF4 Polyclonal antibody (16020-1-AP)

    WB analysis of HeLa using 16020-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 16020-1-AP (ELF4 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of HeLa cells using ELF4 Polyclonal antibody (16020-1-AP)

    IP experiment of HeLa using 16020-1-AP

    IP result of anti-ELF4 (IP:16020-1-AP, 4ug; Detection:16020-1-AP 1:2000) with HeLa cells lysate 1040 ug.

  • Immunoprecipitation (IP) experiment of K-562 cells using ELF4 Polyclonal antibody (16020-1-AP)

    IP experiment of K-562 using 16020-1-AP

    IP result of anti-ELF4 (IP:16020-1-AP, 4ug; Detection:16020-1-AP 1:2000) with K-562 cells lysate 1760 ug.

ELF4 Polyclonal antibody

ELF4 Polyclonal Antibody for IP, ELISA
Cat No. 16020-1-AP
Publications(1)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, ELISA

Synonyms

E74 like factor 4

Formulation:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Positive IP detected inK-562 cells, HeLa cells

Recommended dilution

ApplicationDilution
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

16020-1-AP targets ELF4 in WB, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag8836

Product name: Recombinant human ELF4 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 1-301 aa of BC017194

Sequence: MAITLQPSDLIFEFASNGMDDDIHQLEDPSVFPAVIVEQVPYPDLLHLYSGLELDDVHNGIITDGTLCMTQDQILEGSFLLTDDNEATSHTMSTAEVLLNMESPSDILDEKQIFSTSEMLPDSDPAPAVTLPNYLFPASEPDALNRAGDTSDQEGHSLEEKASREESAKKTGKSKKRIRKTKGNRSTSPVTDPSIPIRKKSKDGKGSTIYLWEFLLALLQDRNTCPKYIKWTQREKGIFKLVDSKAVSKLWGKQKNKPDMNYETMGRALRYYYQRGILAKVEGQRLVYQFKEMPKDLVVIE

相同性解析による交差性が予測される生物種
Full Name E74-like factor 4 (ets domain transcription factor)
Calculated molecular weight 663 aa, 71 kDa
Observed molecular weight71 kDa, 90~100 kDa
GenBank accession numberBC017194
Gene Symbol ELF4
Gene ID (NCBI) 2000
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ99607
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

ELF4 (E74-like ETS transcription factor 4), also widely known as MEF (Myeloid Elf-1-like factor), is a protein-coding gene in humans located on the X chromosome. It encodes a sequence-specific DNA-binding transcription factor that belongs to the evolutionary conserved ETS family. ELF4 is abundantly expressed in normal human hematopoietic tissues. It modulates the cell cycle, ensuring a balanced transition of hematopoietic stem cells and endothelial cells between a resting (quiescent) phase and active cell division (PMID: 40083328). IP analysis detected bands at 71 kDa, while the 90~100 kDa band could be post-translational modification.

Protocols

Product Specific Protocols
IP protocol for ELF4 antibody 16020-1-APDownload protocol
WB protocol for ELF4 antibody 16020-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Nat Immunol

Human autoinflammatory disease reveals ELF4 as a transcriptional regulator of inflammation.

Authors - Paul M Tyler
  • KO Validated
View Article
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