Validation Data Gallery
Tested Applications
| Positive WB detected in | A2780 cells, human brain tissue, human liver tissue |
| Positive IP detected in | A2780 cells |
| Positive IF/ICC detected in | MCF-7 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 2 publications below |
| WB | See 5 publications below |
| IF | See 1 publications below |
| ChIP | See 1 publications below |
Product Information
12499-1-AP targets ELF2 in WB, IF/ICC, IP, ChIP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, goat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag3170 Product name: Recombinant human ELF2 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-353 aa of BC034951 Sequence: MATSLHEGPTNQLDLLIRAVEASVHSSNAHCTDKTIEAAEALLHMESPTCLRDSRSPVEVFVPPCVSTPEFIHAAMRPDVITETVVEVSTEESEPMDTSPIPTSPDSHEPMKKKKVGRKPKTQQSPISNGSPELGIKKKPREGKGNTTYLWEFLLDLLQDKNTCPRYIKWTQREKGIFKLVDSKAVSKLWGKHKNKPDMNYETMGRALRYYYQRGILAKVEGQRLVYQFKDMPKNIVVIDDDKSETCNEDLAGTTDEKSLERVSLSAESLLKAASSVRSGKNSSPINCSRAEKGVARVVNITSPGHDASSRSPTTTASVSATAAPRTVRVAMQVPVVMTSLGQKISTVAVQSV 相同性解析による交差性が予測される生物種 |
| Full Name | E74-like factor 2 (ets domain transcription factor) |
| Calculated molecular weight | 533 aa, 57 kDa |
| Observed molecular weight | 63-64 kDa, 56-57 kDa |
| GenBank accession number | BC034951 |
| Gene Symbol | ELF2 |
| Gene ID (NCBI) | 1998 |
| RRID | AB_10642956 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q15723 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Ets-1 is the prototype member of a family of genes firstly identified on the basis of homology to the v-Ets oncogene isolated from the E26 erythroblastosis virus. Members of the Ets gene family have varied patterns of tissue expression, and share a highly conserved carboxy terminal domain containing a sequence related to the SV40 large T antigen nuclear localization signal sequence. This conserved domain is essential for Ets-1 binding to DNA and is likely to be responsible for the DNA binding activity of all members of the Ets gene family. ELF2 belongs to the Ets-1 family. ELF2 exists several isoforms and the range of molucular weight of isoform is about 54-66 kDa.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for ELF2 antibody 12499-1-AP | Download protocol |
| IP protocol for ELF2 antibody 12499-1-AP | Download protocol |
| WB protocol for ELF2 antibody 12499-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Front Cell Dev Biol A Regulatory Circuit Orchestrated by Novel-miR-3880 Modulates Mammary Gland Development.
| ||
Cell Biol Int Inhibition of the miR-193b-3p protects against oxidized low-density lipoprotein-induced HUVECs injury by upregulating ALDH2. | ||
Ren Fail ATF4-dependent heme-oxygenase-1 attenuates diabetic nephropathy by inducing autophagy and inhibiting apoptosis in podocyte. | ||
Nat Commun Allelic effects on KLHL17 expression underlie a pancreatic cancer genome-wide association signal at chr1p36.33 | ||
medRxiv Allelic effects on KLHL17 expression likely mediated by JunB/D underlie a PDAC GWAS signal at chr1p36.33 |