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Validation Data Gallery

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  • Western Blot (WB) analysis of U2OS cells using ELAVL4 Monoclonal antibody (67835-1-Ig)

    WB analysis of U2OS using 67835-1-Ig (same clone as 67835-1-PBS)

    U2OS cells were subjected to SDS PAGE followed by western blot with 67835-1-Ig (ELAVL4 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67835-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of SH-SY5Y cells using ELAVL4 Monoclonal antibody (67835-1-Ig)

    WB analysis of SH-SY5Y using 67835-1-Ig (same clone as 67835-1-PBS)

    SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 67835-1-Ig (ELAVL4 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67835-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using ELAVL4 Monoclonal antibody (67835-1-Ig)

    WB analysis using 67835-1-Ig (same clone as 67835-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67835-1-Ig (ELAVL4 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67835-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using ELAVL4 Monoclonal antibody (67835-1-Ig)

    IHC staining of mouse brain using 67835-1-Ig (same clone as 67835-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 67835-1-Ig (ELAVL4 antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67835-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using ELAVL4 Monoclonal antibody (67835-1-Ig)

    IHC staining of mouse brain using 67835-1-Ig (same clone as 67835-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 67835-1-Ig (ELAVL4 antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67835-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of U2OS cells using ELAVL4 Monoclonal antibody (67835-1-Ig)

    IF Staining of U2OS using 67835-1-Ig (same clone as 67835-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed U2OS cells using ELAVL4 antibody (67835-1-Ig, Clone: 1B1F5 ) at dilution of 1:800 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (RGAM002), CL594-Phalloidin (red). This data was developed using the same antibody clone with 67835-1-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of U2OS cells using ELAVL4 Monoclonal antibody (67835-1-Ig)

    FC experiment of U2OS using 67835-1-Ig (same clone as 67835-1-PBS)

    1X10^6 U2OS cells were intracellularly stained with 0.4 ug Anti-Human ELAVL4 (67835-1-Ig, Clone:1B1F5) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer. This data was developed using the same antibody clone with 67835-1-PBS in a different storage buffer formulation.

ELAVL4 Monoclonal antibody, PBS Only

ELAVL4 Monoclonal Antibody for WB, IHC, IF/ICC, FC (Intra), Indirect ELISA
Cat No. 67835-1-PBS
Clone No.1B1F5

Host / Isotype

Mouse / IgG2b

Reactivity

human, mouse, rat, pig, rabbit

Applications

WB, IHC, IF/ICC, FC (Intra), Indirect ELISA

Synonyms

HUD, 1B1F5, ELAV like protein 4, ELAV-like protein 4, Hu C/D

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

67835-1-PBS targets ELAVL4 in WB, IHC, IF/ICC, FC (Intra), Indirect ELISA applications and shows reactivity with human, mouse, rat, pig, rabbit samples.

Tested Reactivity human, mouse, rat, pig, rabbit
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen

CatNo: Ag3678

Product name: Recombinant human ELAVL4 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-366 aa of BC036071

Sequence: MVMIISTMEPQVSNGPTSNTSNGPSSNNRNCPSPMQTGATTDDSKTNLIVNYLPQNMTQEEFRSLFGSIGEIESCKLVRDKITGQSLGYGFVNYIDPKDAEKAINTLNGLRLQTKTIKVSYARPSSASIRDANLYVSGLPKTMTQKELEQLFSQYGRIITSRILVGQVTGVSRGVGFIRFDKRIEAEEAIKGLNGQKPSGATEPITVKFANNPSQKSSQALLSQLYQSPNRRYPGPLHHQAQRFRLDNLLNMAYGVKRFSPITIDGMTSLVGMNIPGHTGTGWCIFVYNLSPDSDESVLWQLFGPFGAVNNVKVIRDFNTNKCKGFGFVTMTNYDEAAMAITSLNGYRLGDRVLQVSFKTNKAHKS

相同性解析による交差性が予測される生物種
Full Name ELAV (embryonic lethal, abnormal vision, Drosophila)-like 4 (Hu antigen D)
Calculated molecular weight 380 aa, 42 kDa
Observed molecular weight32-40 kDa
GenBank accession numberBC036071
Gene Symbol ELAVL4
Gene ID (NCBI) 1996
RRIDAB_2918597
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein A purification
UNIPROT IDP26378
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

ELAVL4, also named as HuD, is a 380 amino acid protein. The full name is neuronal embryonic lethal altered visual system-like(ELAVL) RNA-binding protein. ELAVL4 is expressed in brain and localized in the nucleus. PC12 cells expressing T7±HuD were treated with thio-specific (BMH) or amine-specific (DSS) chemical crosslinking reagents, and the resultant crosslinked complexes in the cell extracts were analyzed by immunoblotting with anti-T7 antibody. In addition to the monomeric HuD of 41 kDa, three specific crosslinked complexes could be detected using either of the crosslinkers. Two of the complexes had molecular masses of ~80 and 120 kDa, and were likely to be the dimer and trimer of HuD, respectively. (PMID: 12384599 )

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