Validation Data Gallery
Tested Applications
| Positive WB detected in | SH-SY5Y cells, mouse cerebellum tissue |
| Positive IP detected in | mouse cerebellum tissue |
| Positive IHC detected in | human brain tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 2 publications below |
| WB | See 10 publications below |
| IHC | See 2 publications below |
| IF | See 6 publications below |
| IP | See 2 publications below |
| CoIP | See 1 publications below |
| RIP | See 3 publications below |
Product Information
14008-1-AP targets ELAVL2 in WB, IHC, IF, IP, CoIP, RIP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat, chicken |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag5105 Product name: Recombinant human ELAVL2 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 5-346 aa of BC030692 Sequence: LSNGPTCNNTANGPTTINNNCSSPVDSGNTEDSKTNLIVNYLPQNMTQEELKSLFGSIGEIESCKLVRDKITGQSLGYGFVNYIDPKDAEKAINTLNGLRLQTKTIKVSYARPSSASIRDANLYVSGLPKTMTQKELEQLFSQYGRIITSRILVDQVTGISRGVGFIRFDKRIEAEEAIKGLNGQKPPGATEPITVKFANNPSQKTNQAILSQLYQSPNRRYPGPLAQQAQRFRFSPMTIDGMTSLAGINIPGHPGTGWCIFVYNLAPDADESILWQMFGPFGAVTNVKVIRDFNTNKCKGFGFVTMTNYDEAAMAIASLNGYRLGDRVLQVSFKTNKTHKA 相同性解析による交差性が予測される生物種 |
| Full Name | ELAV (embryonic lethal, abnormal vision, Drosophila)-like 2 (Hu antigen B) |
| Calculated molecular weight | 39 kDa |
| Observed molecular weight | 39 kDa |
| GenBank accession number | BC030692 |
| Gene Symbol | ELAVL2 |
| Gene ID (NCBI) | 1993 |
| RRID | AB_2096356 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q12926 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
The ELAV (embryonic lethal abnormal visual system) gene of D. Melanogaste is the the first identified member of a family of neuronal RNA binding proteins that is conserved in metazoans. The proteins in this family contain three RNA Recognition Motifs (RRM), with a hinge region separating the second and third RRMs and an optional non-conserved N-terminal region. The hinge includes signals essential for nuclear export and subcellular localization [PMID:9874760]. Elav is expressed specifically in the neurons and is required for the correct development and maintenance of the nervous system. ELAVL2 can binds RNA, and recognize a GAAA motif. In addition, it can bind to its own 3'-UTR, the FOS 3'-UTR and the ID 3'-UTR[PMID:18715504,21695151].
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for ELAVL2 antibody 14008-1-AP | Download protocol |
| IP protocol for ELAVL2 antibody 14008-1-AP | Download protocol |
| WB protocol for ELAVL2 antibody 14008-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Mol Cell RAPIDASH: Tag-free enrichment of ribosome-associated proteins reveals composition dynamics in embryonic tissue, cancer cells, and macrophages | ||
Sci Adv Mature mRNA processing that deletes 3' end sequences directs translational activation and embryonic development | ||
Nucleic Acids Res HuB and HuD repress telomerase activity by dissociating HuR from TERC.
| ||
EMBO Rep ELAVL2-directed RNA regulatory network drives the formation of quiescent primordial follicles. | ||
Cell Rep miR-302 Is Required for Timing of Neural Differentiation, Neural Tube Closure, and Embryonic Viability. | ||
Hum Mol Genet ELAVL2-regulated transcriptional and splicing networks in human neurons link neurodevelopment and autism.
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