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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using ELAC2 Recombinant antibody (83468-6-RR)

    WB analysis using 83468-6-RR (same clone as 83468-6-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 83468-6-RR (ELAC2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83468-6-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of MCF-7 cells using ELAC2 Recombinant antibody (83468-6-RR)

    WB analysis of MCF-7 using 83468-6-RR (same clone as 83468-6-PBS)

    WB result of ELAC2 antibody (83468-6-RR; 1:600; incubated at room temperature for 1.5 hours) with sh-Control and sh-ELAC2 transfected MCF-7 cells. This data was developed using the same antibody clone with 83468-6-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of A431 cells using ELAC2 Recombinant antibody (83468-6-RR)

    FC experiment of A431 using 83468-6-RR (same clone as 83468-6-PBS)

    1x10^6 A431 cells were intracellularly stained with 0.25 ug ELAC2 Recombinant antibody (83468-6-RR, Clone:240487B6) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 83468-6-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 83468-6-RR

    Affinity and Kinetic Characterization of 83468-6-RR

    Biolayer interferometry (BLl) kinetic assays of 83468-6-RR against Human ELAC2 were performed. The affinity constant is below 1 pM.

  • Featured Product
  • KD/KO Validated

ELAC2 Recombinant monoclonal antibody, PBS Only

ELAC2 Uni-rAb® Recombinant Antibody for WB, FC (Intra), ELISA
Cat No. 83468-6-PBS
Clone No.240487B6

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, FC (Intra), ELISA

Synonyms

240487B6, EC:3.1.26.11, elaC homolog 2 (E. coli), ElaC homolog protein 2, ELC2

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

83468-6-PBS targets ELAC2 in WB, FC (Intra), ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen

CatNo: Ag0105

Product name: Recombinant human ELAC2 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 86-385 aa of BC004158

Sequence: NCGEGVQRLMQEHKLKVARLDNIFLTRMHWSNVGGLSGMILTLKETGLPKCVLSGPPQLEKYLEAIKIFSGPLKGIELAVRPHSAPEYEDETMTVYQIPIHSEQRRGKHQPWQSPERPLSRLSPERSSDSELNENEPHLPHGVSQRRGVRDSSLVVAFICKLHLKRGNFLVLKAKEMGLPVGTAAIAPIIAAVKDGKSITHEGREILAEELCTPPDPGAAFVVVECPDESFIQPICENATFQRYQGKADAPVALVVHMAPASVLVDSRYQQWMERFGPDTQHLVLNENCASVHNLRSHKI

相同性解析による交差性が予測される生物種
Full Name elaC homolog 2 (E. coli)
Calculated molecular weight 92 kDa
Observed molecular weight 97 kDa
GenBank accession numberBC004158
Gene Symbol ELAC2
Gene ID (NCBI) 60528
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein A purfication
UNIPROT IDQ9BQ52
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

Putative prostate cancer susceptibility protein (ELAC2) is encoded by HPC2/ELAC2 gene. HPC2 is a potential prostate cancer predisposition gene playing a role in genetic susceptibility to prostate cancer.It is a zinc phosphodiesterase, which displays some tRNA 3'-processing endonuclease activity and probably involved in tRNA maturation, by removing a 3'-trailer from precursor tRNA.Defects in ELAC2 may be a cause of susceptibility to prostate cancer (PC).

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