Validation Data Gallery
Tested Applications
| Positive WB detected in | mouse pancreas tissue, rat pancreas tissue |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
15737-1-AP targets ELA3A in WB, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag8536 Product name: Recombinant human ELA3A protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 22-270 aa of BC008383 Sequence: SSHSSSRVVHGEDAVPYSWPWQVSLQYEKSGSFYHTCGGSLIAPDWVVTAGHCISRDLTYQVVLGEYNLAVKEGPEQVIPINSEELFVHPLWNRSCVACGNDIALIKLSRSAQLGDAVQLASLPPAGDILPNKTPCYITGWGRLYTNGPLPDKLQQARLPVVDYKHCSRWNWWGSTVKKTMVCAGGYIRSGCNGDSGGPLNCPTEDGGWQVHGVTSFVSGFGCNFIWKPTVFTRVSAFIDWIEETIASH 相同性解析による交差性が予測される生物種 |
| Full Name | elastase 3A, pancreatic |
| Calculated molecular weight | 270 aa, 29 kDa |
| Observed molecular weight | 29 kDa |
| GenBank accession number | BC008383 |
| Gene Symbol | ELA3A |
| Gene ID (NCBI) | 10136 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P09093 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
ELA3A, also known as CELA3A (Chymotrypsin Like Elastase 3A), is synthesized in the pancreas and secreted into the intestine as an inactive precursor, or zymogen, to prevent it from digesting the pancreas itself. Structurally, the mature protein is 270 amino acids long with a molecular mass of approximately 29.5 kDa . It contains a conserved trypsin-like serine protease domain (Peptidase S1) characteristic of its function.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for ELA3A antibody 15737-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |