Validation Data Gallery

Filter: AllWBIHCIF/ICC

WB analysis using 11309-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 11309-1-AP (EIF5A antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.
WB analysis using 11309-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 11309-1-AP (EIF5A antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.
WB analysis using 11309-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 11309-1-AP (EIF5A antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.
WB analysis of HeLa using 11309-1-AP
HeLa cells were subjected to SDS PAGE followed by western blot with 11309-1-AP (EIF5A antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
IHC staining of mouse testis using 11309-1-AP
Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 11309-1-AP (EIF5A antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse testis using 11309-1-AP
Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 11309-1-AP (EIF5A antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IF Staining of HeLa using 11309-1-AP
Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using EIF5A antibody (11309-1-AP) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), CL594-Phalloidin (red).

Featured Product
KD/KO Validated

EIF5A Polyclonal antibody

EIF5A Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Cat No. 11309-1-AP

Publications(7)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, ELISA

Synonyms

eIF-5A, eIF-4D, eIF 5A1, eIF 5A 1, EIF 5A

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

Conjugate: Unconjugated

Unconjugated

Size/Concentration:

-/ -

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Overview

Tested Applications
Product Information
Publications (7)
Protocols

データシート［PDF］を印刷

MSDS

Tested Applications

Positive WB detected in	HeLa cells, NIH/3T3 cells, PC-3 cells, SH-SY5Y cells, mouse brain tissue
Positive IHC detected in	mouse testis tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	HeLa cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:5000-1:50000
Immunohistochemistry (IHC)	IHC : 1:50-1:500
Immunofluorescence (IF)/ICC	IF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 1 publications below
WB	See 5 publications below
IHC	See 1 publications below
IF	See 1 publications below
IP	See 1 publications below

Product Information

11309-1-AP targets EIF5A in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human, mouse
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag1849 Product name: Recombinant human EIF5A protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-154 aa of BC001832 Sequence: MADDLDFETGDAGASATFPMQCSALRKNGFVVLKGRPCKIVEMSTSKTGKHGHAKVHLVGIDIFTGKKYEDICPSTHNMDVPNIKRNDFQLIGIQDGYLSLLQDSGEVREDLRLPEGDLGKEIEQKYDCGEEILITVLSAMTEEAAVAIKAMAK 相同性解析による交差性が予測される生物種
Full Name	eukaryotic translation initiation factor 5A
Calculated molecular weight	18 kDa
Observed molecular weight	18 kDa
GenBank accession number	BC001832
Gene Symbol	EIF5A
Gene ID (NCBI)	1984
RRID	AB_2262001
Conjugate	Unconjugated
Form
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	P63241
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

Translation of messenger RNA (mRNA) to protein in eukaryotesis a crucial process in protein biosynthesis, in which initiation of translation involves interaction of different eukaryotic translation initiation factors (eIFs), ribosome subunits and mRNAs. Eukaryotic translation initiation factor 5A (EIF5A) is one of the eIFs involved in translation initiation. EIF5A has a essential role in cell viability and the only protein known to contain the amino acid residue hypusine, formed by post-translational modification of a specific lysine residue. Firstly identified as a translation initiation factor, it also has a function in the elongation step of translation.

Protocols

Product Specific Protocols
IF protocol for EIF5A antibody 11309-1-AP	Download protocol
IHC protocol for EIF5A antibody 11309-1-AP	Download protocol
WB protocol for EIF5A antibody 11309-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human	WB,IHC	J Cancer EIF5A expression and its role as a potential diagnostic biomarker in hepatocellular carcinoma. Authors - Linjing Li View Article
		BMC Cancer Multi-omics analysis reveals the impact of YAP/TEAD4-mediated EIF5A1 expression on mitochondrial apoptosis and bladder cancer progression Authors - Kun-Peng Li View Article
human	WB	Oncol Lett SHCBP1 contributes to the proliferation and self‑renewal of cervical cancer cells and activation of the NF‑κB signaling pathway through EIF5A Authors - Boya Deng View Article
mouse	IF	J Ovarian Res Proteomic analysis implicates that postovulatory aging leads to aberrant gene expression, biosynthesis, RNA metabolism and cell cycle in mouse oocytes Authors - Chuanxin Zhang View Article
human	WB	Oral Dis ZNF331 Represses the Proliferation of Head and Neck Squamous Cell Carcinoma via Co-Repressor TRIM28 Authors - Ju Li View Article
human	WB,IP	Cancer Res A Genome-Wide Synthetic Lethal Screen Identifies Spermidine Synthase as a Target to Enhance Erdafitinib Efficacy in FGFR-Mutant Bladder Cancer Authors - Yanchao Yu View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Validation Data Gallery

WB analysis using 11309-1-AP

WB analysis using 11309-1-AP

WB analysis using 11309-1-AP

WB analysis of HeLa using 11309-1-AP

IHC staining of mouse testis using 11309-1-AP

IHC staining of mouse testis using 11309-1-AP

IF Staining of HeLa using 11309-1-AP

EIF5A Polyclonal antibody

EIF5A Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

-/ -

Tested Applications

Recommended dilution

Published Applications

Product Information

Background Information

Protocols

Publications

EIF5A expression and its role as a potential diagnostic biomarker in hepatocellular carcinoma.

Multi-omics analysis reveals the impact of YAP/TEAD4-mediated EIF5A1 expression on mitochondrial apoptosis and bladder cancer progression

SHCBP1 contributes to the proliferation and self‑renewal of cervical cancer cells and activation of the NF‑κB signaling pathway through EIF5A

Proteomic analysis implicates that postovulatory aging leads to aberrant gene expression, biosynthesis, RNA metabolism and cell cycle in mouse oocytes

ZNF331 Represses the Proliferation of Head and Neck Squamous Cell Carcinoma via Co-Repressor TRIM28

A Genome-Wide Synthetic Lethal Screen Identifies Spermidine Synthase as a Target to Enhance Erdafitinib Efficacy in FGFR-Mutant Bladder Cancer