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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using EIF4G3 Polyclonal antibody (11281-1-AP)

    WB analysis using 11281-1-AP (same clone as 11281-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 11281-1-AP (EIF4G3 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11281-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HEK-293 cells using EIF4G3 Polyclonal antibody (11281-1-AP)

    WB analysis of HEK-293 using 11281-1-AP (same clone as 11281-1-PBS)

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 11281-1-AP (EIF4G3 Antibody) at dilution of 1:600 incubated at 4 degree celsius over night. This data was developed using the same antibody clone with 11281-1-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of HeLa cells using EIF4G3 Polyclonal antibody (11281-1-AP)

    IP experiment of HeLa using 11281-1-AP (same clone as 11281-1-PBS)

    IP result of anti-EIF4G3 (IP:11281-1-AP, 4ug; Detection:11281-1-AP 1:500) with HeLa cells lysate 4000ug. This data was developed using the same antibody clone with 11281-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human gliomas tissue using EIF4G3 Polyclonal antibody (11281-1-AP)

    IHC staining of human gliomas using 11281-1-AP (same clone as 11281-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human gliomas using 11281-1-AP (EIF4G3 antibody) at dilution of 1:10 (under 10x lens). This data was developed using the same antibody clone with 11281-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using EIF4G3 Polyclonal antibody (11281-1-AP)

    IF Staining of HeLa using 11281-1-AP (same clone as 11281-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using EIF4G3 antibody (11281-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 11281-1-PBS in a different storage buffer formulation.

EIF4G3 Polyclonal antibody, PBS Only

EIF4G3 Polyclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA
Cat No. 11281-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, IP, Indirect ELISA

Synonyms

eIF4GII, eIF-4-gamma II, eIF-4-gamma 3, eIF-4G 3, eIF4G 3

Formulation:  PBS Only
PBS, Azide, Glycerol
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

11281-1-PBS targets EIF4G3 in WB, IHC, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag1811

Product name: Recombinant human EIF4G3 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 197-515 aa of BC030578

Sequence: SQVPEHSPVVYGTVESAHLAASTPVTAASDQKQEEKPKPDPVLKSPSPVLRLVLSGEKKEQEGQTSETTAIVSIAELPLPPSPTTVSSVARSTIAAPTSSALSSQPIFTTAIDDRCELSSPREDTIPIPSLTSCTETSDPLPTNENDGDICKKPCSVAPNDIPLVSSTNLINEINGVSEKLSATESIVEIVKQEVLPLTLELEILENPPEEMKLECIPAPITPSTVPSFPPTPPTPPASPPHTPVIVPAAATTVSSPSAAITVQRVLEEDESIRTCLSEDAKEIQNKIEVEADGQTEEILDSQNLNSRRSPVPETSNEC

相同性解析による交差性が予測される生物種
Full Name eukaryotic translation initiation factor 4 gamma, 3
Calculated molecular weight 177 kDa
Observed molecular weight 70 kDa, 250 kDa
GenBank accession numberBC030578
Gene Symbol EIF4G3
Gene ID (NCBI) 8672
RRIDAB_2877754
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDO43432
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

Eukaryotic translation initiation factor 4 gamma, 3 (EIF4G3) may be involved in the recognition of the mRNA cap. The protein migrated at approximately 70 kDa and/or 250 kDa in SDS-PAGE.

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