Validation Data Gallery

Filter: AllWBIPIHCIF/ICC

WB analysis using 17728-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 17728-1-AP (DAP5 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.
WB analysis of HeLa using 17728-1-AP
HeLa cells were subjected to SDS PAGE followed by western blot with 17728-1-AP (DAP5 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of MCF-7 using 17728-1-AP
MCF7 cells were subjected to SDS PAGE followed by western blot with 17728-1-AP (DAP5 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.
IP experiment of MCF-7 using 17728-1-AP
IP result of anti-eIF4G2/DAP5 (IP:17728-1-AP, 4ug; Detection:17728-1-AP 1:400) with MCF-7 cells lysate 3200ug.
IHC staining of human skin cancer using 17728-1-AP
Immunohistochemical analysis of paraffin-embedded skin cancer slide using 17728-1-AP (eIF4G2/DAP5 antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human skin cancer using 17728-1-AP
Immunohistochemical analysis of paraffin-embedded skin cancer slide using 17728-1-AP (eIF4G2/DAP5 antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human prostate cancer using 17728-1-AP
Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 17728-1-AP (eIF4G2/DAP5 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human prostate cancer using 17728-1-AP
Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 17728-1-AP (eIF4G2/DAP5 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IF Staining of HepG2 using 17728-1-AP
Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using eIF4G2/DAP5 antibody (17728-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2).

eIF4G2/DAP5 Polyclonal antibody

eIF4G2/DAP5 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Cat No. 17728-1-AP

Publications(5)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, IP, RIP, ELISA

Synonyms

EIF4G2, Death-associated protein 5, Death associated protein 5, DAP-5, DAP5

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

Conjugate: Unconjugated

Unconjugated

Size/Concentration:

-/ -

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Overview

Tested Applications
Product Information
Publications (5)
Protocols

データシート［PDF］を印刷

MSDS

Tested Applications

Positive WB detected in	Jurkat cells, HeLa cells, MCF-7 cells, Neuro-2a cells
Positive IP detected in	MCF-7 cells
Positive IHC detected in	human prostate cancer tissue, human skin cancer tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	HepG2 cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:1000-1:4000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:50-1:500
Immunofluorescence (IF)/ICC	IF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 1 publications below
WB	See 5 publications below
RIP	See 1 publications below

Product Information

17728-1-AP targets eIF4G2/DAP5 in WB, IHC, IF/ICC, IP, RIP, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity	human, mouse
Cited Reactivity	human
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag12125 Product name: Recombinant human EIF4G2 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 563-907 aa of BC043149 Sequence: EAVNGVREMRAPKHFLPEMLSKVIILSLDRSDEDKEKASSLISLLKQEGIATSDNFMQAFLNVLDQCPKLEVDIPLVKSYLAQFAARAIISELVSISELAQPLESGTHFPLFLLCLQQLAKLQDREWLTELFQQSKVNMQKMLPEIDQNKDRMLEILEGKGLSFLFPLLKLEKELLKQIKLDPSPQTIYKWIKDNISPKLHVDKGFVNILMTSFLQYISSEVNPPSDETDSSSAPSKEQLEQEKQLLLSFKPVMQKFLHDHVDLQVSALYALQVHCYNSNFPKGMLLRFFVHFYDMEIIEEEAFLAWKEDITQEFPGKGKALFQVNQWLTWLETAEEEESEEEAD 相同性解析による交差性が予測される生物種
Full Name	eukaryotic translation initiation factor 4 gamma, 2
Calculated molecular weight	907 aa, 102 kDa
Observed molecular weight	98-102 kDa
GenBank accession number	BC043149
Gene Symbol	EIF4G2
Gene ID (NCBI)	1982
RRID	AB_2095895
Conjugate	Unconjugated
Form
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	P78344
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Protocols

Product Specific Protocols
IF protocol for eIF4G2/DAP5 antibody 17728-1-AP	Download protocol
IHC protocol for eIF4G2/DAP5 antibody 17728-1-AP	Download protocol
IP protocol for eIF4G2/DAP5 antibody 17728-1-AP	Download protocol
WB protocol for eIF4G2/DAP5 antibody 17728-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human	WB	Nat Commun PMID: 31792210 Authors - Sunwang Xu View Article
human	WB	Shock miR-379-5P INHIBITION ENHANCES INTESTINAL EPITHELIAL PROLIFERATION AND BARRIER FUNCTION RECOVERY AFTER ISCHEMIA/REPERFUSION BY TARGETING EIF4G2 Authors - Zirui Jia View Article
human	WB,RIP	J Proteome Res EIF4G2 Promotes Hepatocellular Carcinoma Progression via IRES-dependent PLEKHA1 Translation Regulation Authors - Manman Li KD Validated View Article
human	WB	Nat Commun The protein circPETH-147aa regulates metabolic reprogramming in hepatocellular carcinoma cells to remodel immunosuppressive microenvironment Authors - Tian Lan View Article
human	WB	Gene The proximity proteome of pre-40S pre-ribosomal particle components PNO1 and NOB1 using turboID proximity labeling technology Authors - Xingyuan Xu View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Validation Data Gallery

WB analysis using 17728-1-AP

WB analysis of HeLa using 17728-1-AP

WB analysis of MCF-7 using 17728-1-AP

IP experiment of MCF-7 using 17728-1-AP

IHC staining of human skin cancer using 17728-1-AP

IHC staining of human skin cancer using 17728-1-AP

IHC staining of human prostate cancer using 17728-1-AP

IHC staining of human prostate cancer using 17728-1-AP

IF Staining of HepG2 using 17728-1-AP

eIF4G2/DAP5 Polyclonal antibody

eIF4G2/DAP5 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

-/ -

Tested Applications

Recommended dilution

Published Applications

Product Information

Protocols

Publications

PMID: 31792210

miR-379-5P INHIBITION ENHANCES INTESTINAL EPITHELIAL PROLIFERATION AND BARRIER FUNCTION RECOVERY AFTER ISCHEMIA/REPERFUSION BY TARGETING EIF4G2

EIF4G2 Promotes Hepatocellular Carcinoma Progression via IRES-dependent PLEKHA1 Translation Regulation

The protein circPETH-147aa regulates metabolic reprogramming in hepatocellular carcinoma cells to remodel immunosuppressive microenvironment

The proximity proteome of pre-40S pre-ribosomal particle components PNO1 and NOB1 using turboID proximity labeling technology