- Featured Product
- KD/KO Validated
EIF4G1 Polyclonal antibody
EIF4G1 Polyclonal Antibody for IF, IHC, IP, WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human and More (1)
Applications
WB, IP, IHC, IF, CoIP, ELISA
Conjugate
Unconjugated
Cat no : 15704-1-AP
Synonyms
Validation Data Gallery
Tested Applications
| Positive WB detected in | BxPC-3 cells, HeLa cells, HepG2 cells |
| Positive IP detected in | HeLa cells |
| Positive IHC detected in | human breast cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF detected in | Hela cells, HepG2 cells, Ethacrynic acid treated HepG2 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:16000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF) | IF : 1:10-1:100 |
| Sample-dependent, check data in validation data gallery | |
Published Applications
| KD/KO | See 1 publications below |
| WB | See 13 publications below |
| IHC | See 4 publications below |
| IF | See 5 publications below |
| CoIP | See 1 publications below |
Product Information
15704-1-AP targets EIF4G1 in WB, IP, IHC, IF, CoIP, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | EIF4G1 fusion protein Ag8342 相同性解析による交差性が予測される生物種 |
| Full Name | eukaryotic translation initiation factor 4 gamma, 1 |
| Calculated molecular weight | 1600 aa, 176 kDa |
| Observed molecular weight | 250 kDa |
| GenBank accession number | BC007788 |
| Gene symbol | EIF4G1 |
| Gene ID (NCBI) | 1981 |
| RRID | AB_2261979 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Eukaryotic cellular messenger RNAs are posttranscriptionally modified by addition of an m(7)GTP moiety to the 5-prime terminus, referred to as a cap. Recognition of the cap structure and unwinding of mRNA secondary structure during the initiation phase of protein synthesis is catalyzed by initiation factors of the eIF4 group. EIF4G1, a subunit of eIF4 gamma, forms various complexes with the other eIF4 polypeptides [PMID: 7601469]. Mutations in the EIF4G1 gene, encoding a component of the eIF4F translation initiation complex, were recently reported as a possible cause for the autosomal dominant form of Parkinson's disease [PMID:22658323]. The calcualted molecular weight of EIF4G1 is 175 kDa, but modified EIF4G1 is about 220-240 kDa. (PMID: 18426977 )
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for EIF4G1 antibody 15704-1-AP | Download protocol |
| IHC protocol for EIF4G1 antibody 15704-1-AP | Download protocol |
| IF protocol for EIF4G1 antibody 15704-1-AP | Download protocol |
| IP protocol for EIF4G1 antibody 15704-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Mol Cell The oncomicropeptide APPLE promotes hematopoietic malignancy by enhancing translation initiation. | ||
J Extracell Vesicles Extracellular vesicles rich in HAX1 promote angiogenesis by modulating ITGB6 translation. | ||
Proc Natl Acad Sci U S A Cellular cap-binding protein, eIF4E, promotes picornavirus genome restructuring and translation. | ||
PLoS Genet SAMHD1 Inhibits LINE-1 Retrotransposition by Promoting Stress Granule Formation. | ||
Diabetes Translational Factor eIF4G1 Regulates Glucose Homeostasis and Pancreatic β-Cell Function.
| ||
J Cell Sci ADAR1 limits stress granule formation through both translation-dependent and translation-independent mechanisms. |
