Validation Data Gallery

Filter: AllWBIPIHCIF/ICC

WB analysis of A549 using 17504-1-AP
A549 cells were subjected to SDS PAGE followed by western blot with 17504-1-AP (EIF4A3 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
WB analysis using 17504-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 17504-1-AP (EIF4A3 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.
WB analysis of mouse thymus using 17504-1-AP
mouse thymus tissue were subjected to SDS PAGE followed by western blot with 17504-1-AP (EIF4A3 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.
WB analysis of A549 using 17504-1-AP
A549 cells were subjected to SDS PAGE followed by western blot with 17504-1-AP (EIF4A3 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.
WB analysis of mouse heart using 17504-1-AP
mouse heart tissue were subjected to SDS PAGE followed by western blot with 17504-1-AP (EIF4A3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of mouse liver using 17504-1-AP
mouse liver tissue were subjected to SDS PAGE followed by western blot with 17504-1-AP (EIF4A3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of HeLa using 17504-1-AP
HeLa cells were subjected to SDS PAGE followed by western blot with 17504-1-AP (EIF4A3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of mouse lung using 17504-1-AP
mouse lung tissue were subjected to SDS PAGE followed by western blot with 17504-1-AP (EIF4A3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of HEK-293 using 17504-1-AP
HEK-293 cells were subjected to SDS PAGE followed by western blot with 17504-1-AP (EIF4A3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of MCF-7 using 17504-1-AP
MCF7 cells were subjected to SDS PAGE followed by western blot with 17504-1-AP (EIF4A3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of mouse thymus using 17504-1-AP
mouse thymus tissue were subjected to SDS PAGE followed by western blot with 17504-1-AP (EIF4A3 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
IP experiment of mouse heart using 17504-1-AP
IP result of anti-EIF4A3 (IP:17504-1-AP, 3ug; Detection:17504-1-AP 1:1000) with mouse heart tissue lysate 5000ug.
IHC staining of human kidney using 17504-1-AP
Immunohistochemical analysis of paraffin-embedded human kidney using 17504-1-AP (EIF4A3 antibody) at dilution of 1:100 (under 40x lens).
IHC staining of human testis using 17504-1-AP
Immunohistochemical analysis of paraffin-embedded human testis using 17504-1-AP (EIF4A3 antibody) at dilution of 1:100 (under 10x lens).
IHC staining of human testis using 17504-1-AP
Immunohistochemical analysis of paraffin-embedded human testis using 17504-1-AP (EIF4A3 antibody) at dilution of 1:100 (under 40x lens).
IHC staining of human brain using 17504-1-AP
Immunohistochemical analysis of paraffin-embedded human brain using 17504-1-AP (EIF4A3 antibody) at dilution of 1:100 (under 10x lens).
IHC staining of human brain using 17504-1-AP
Immunohistochemical analysis of paraffin-embedded human brain using 17504-1-AP (EIF4A3 antibody) at dilution of 1:100 (under 40x lens).
IHC staining of human spleen using 17504-1-AP
Immunohistochemical analysis of paraffin-embedded human spleen using 17504-1-AP (EIF4A3 antibody) at dilution of 1:100 (under 10x lens).
IHC staining of human spleen using 17504-1-AP
Immunohistochemical analysis of paraffin-embedded human spleen using 17504-1-AP (EIF4A3 antibody) at dilution of 1:100 (under 40x lens).
IHC staining of human lung using 17504-1-AP
Immunohistochemical analysis of paraffin-embedded human lung using 17504-1-AP (EIF4A3 antibody) at dilution of 1:100 (under 10x lens).
IHC staining of human lung using 17504-1-AP
Immunohistochemical analysis of paraffin-embedded human lung using 17504-1-AP (EIF4A3 antibody) at dilution of 1:100 (under 40x lens).
IHC staining of human ovary using 17504-1-AP
Immunohistochemical analysis of paraffin-embedded human ovary using 17504-1-AP (EIF4A3 antibody) at dilution of 1:100 (under 10x lens).
IHC staining of human ovary using 17504-1-AP
Immunohistochemical analysis of paraffin-embedded human ovary using 17504-1-AP (EIF4A3 antibody) at dilution of 1:100 (under 40x lens).
IHC staining of human heart using 17504-1-AP
Immunohistochemical analysis of paraffin-embedded human heart using 17504-1-AP (EIF4A3 antibody) at dilution of 1:50 (under 10x lens).
IHC staining of human heart using 17504-1-AP
Immunohistochemical analysis of paraffin-embedded human heart using 17504-1-AP (EIF4A3 antibody) at dilution of 1:50 (under 40x lens).
IHC staining of human kidney using 17504-1-AP
Immunohistochemical analysis of paraffin-embedded human kidney using 17504-1-AP (EIF4A3 antibody) at dilution of 1:50 (under 10x lens).
IHC staining of human kidney using 17504-1-AP
Immunohistochemical analysis of paraffin-embedded human kidney using 17504-1-AP (EIF4A3 antibody) at dilution of 1:50 (under 40x lens).
IHC staining of human kidney using 17504-1-AP
Immunohistochemical analysis of paraffin-embedded human kidney using 17504-1-AP (EIF4A3 antibody) at dilution of 1:100 (under 10x lens).
IF Staining of MCF-7 using 17504-1-AP
Immunofluorescent analysis of MCF-7 cells, using EIF4A3 antibody 17504-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

Featured Product
KD/KO Validated

EIF4A3 Polyclonal antibody

EIF4A3 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Cat No. 17504-1-AP

Publications(58)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, CoIP, RIP, ELISA

Synonyms

EC:3.6.4.13, DEAD box protein 48, DDX48, DDX 48, ATP-dependent RNA helicase eIF4A-3

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

Conjugate: Unconjugated

Unconjugated

Size/Concentration:

-/ -

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Overview

Tested Applications
Product Information
Publications (58)
Protocols

データシート［PDF］を印刷

MSDS

Tested Applications

Positive WB detected in	A549 cells, mouse thymus tissue, mouse heart tissue, mouse lung tissue, HEK-293 cells, mouse liver tissue, HeLa cells, MCF-7 cells, HepG2 cells
Positive IP detected in	mouse heart tissue
Positive IHC detected in	human kidney tissue, human brain tissue, human heart tissue, human lung tissue, human ovary tissue, human spleen tissue, human testis tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	MCF-7 cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:1000-1:4000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:20-1:200
Immunofluorescence (IF)/ICC	IF/ICC : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 6 publications below
WB	See 41 publications below
IHC	See 5 publications below
IF	See 12 publications below
IP	See 3 publications below
CoIP	See 4 publications below
RIP	See 16 publications below

Product Information

17504-1-AP targets EIF4A3 in WB, IHC, IF/ICC, IP, CoIP, RIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human, mouse, rat
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag11701 Product name: Recombinant human EIF4A3 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-411 aa of BC003662 Sequence: MATTATMATSGSARKRLLKEEDMTKVEFETSEEVDVTPTFDTMGLREDLLRGIYAYGFEKPSAIQQRAIKQIIKGRDVIAQSQSGTGKTATFSISVLQCLDIQVRETQALILAPTRELAVQIQKGLLALGDYMNVQCHACIGGTNVGEDIRKLDYGQHVVAGTPGRVFDMIRRRSLRTRAIKMLVLDEADEMLNKGFKEQIYDVYRYLPPATQVVLISATLPHEILEMTNKFMTDPIRILVKRDELTLEGIKQFFVAVEREEWKFDTLCDLYDTLTITQAVIFCNTKRKVDWLTEKMREANFTVSSMHGDMPQKERESIMKEFRSGASRVLISTDVWARGLDVPQVSLIINYDLPNNRELYIHRIGRSGRYGRKGVAINFVKNDDIRILRDIEQYYSTQIDEMPMNVADLI 相同性解析による交差性が予測される生物種
Full Name	eukaryotic translation initiation factor 4A, isoform 3
Calculated molecular weight	47 kDa
Observed molecular weight	47 kDa
GenBank accession number	BC003662
Gene Symbol	EIF4A3
Gene ID (NCBI)	9775
RRID	AB_2097393
Conjugate	Unconjugated
Form
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	P38919
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

EIF4A3 is a component of the exon junction complex (EJC), which assembles near exon-exon junctions of mRNAs as a result of splicing. EJC proteins involves in postsplicing events, including mRNA export, cytoplasmic localization, and nonsense-mediated decay. Its RNA-dependent ATPase and RNA-helicase activities are induced by CASC3, but abolished in presence of the MAGOH/RBM8A heterodimer, thereby trapping the ATP-bound EJC core onto spliced mRNA in a stable conformation. Besides, it involved in translational enhancement of spliced mRNAs after formation of the 80S ribosome complex and binds spliced mRNA in sequence-independent manner, 20-24 nucleotides upstream of mRNA exon-exon junctions

Protocols

Product Specific Protocols
IF protocol for EIF4A3 antibody 17504-1-AP	Download protocol
IHC protocol for EIF4A3 antibody 17504-1-AP	Download protocol
IP protocol for EIF4A3 antibody 17504-1-AP	Download protocol
WB protocol for EIF4A3 antibody 17504-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
	WB	J Hematol Oncol METTL16 promotes liver cancer stem cell self-renewal via controlling ribosome biogenesis and mRNA translation Authors - Meilin Xue View Article
human	WB	Cell Metab NEAT1 is essential for metabolic changes that promote breast cancer growth and metastasis. Authors - Mi Kyung Park View Article
		Cell Res YTHDF3 facilitates translation and decay of N(6)-methyladenosine-modified RNA. Authors - Hailing Shi View Article
human	WB,RIP,CoIP	J Exp Clin Cancer Res Hypoxia-enhanced YAP1-EIF4A3 interaction drives circ_0007386 circularization by competing with CRIM1 pre-mRNA linear splicing and promotes non-small cell lung cancer progression Authors - Lixia Li View Article
human	IF,WB	Cell Death Differ eIF4A3 regulates the TFEB-mediated transcriptional response via GSK3B to control autophagy. Authors - Despoina Sakellariou KD Validated View Article
human	WB	Mol Cancer Long noncoding RNA ZFAS1 promoting small nucleolar RNA-mediated 2'-O-methylation via NOP58 recruitment in colorectal cancer. Authors - Huizhe Wu View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Validation Data Gallery

WB analysis of A549 using 17504-1-AP

WB analysis using 17504-1-AP

WB analysis of mouse thymus using 17504-1-AP

WB analysis of A549 using 17504-1-AP

WB analysis of mouse heart using 17504-1-AP

WB analysis of mouse liver using 17504-1-AP

WB analysis of HeLa using 17504-1-AP

WB analysis of mouse lung using 17504-1-AP

WB analysis of HEK-293 using 17504-1-AP

WB analysis of MCF-7 using 17504-1-AP

WB analysis of mouse thymus using 17504-1-AP

IP experiment of mouse heart using 17504-1-AP

IHC staining of human kidney using 17504-1-AP

IHC staining of human testis using 17504-1-AP

IHC staining of human testis using 17504-1-AP

IHC staining of human brain using 17504-1-AP

IHC staining of human brain using 17504-1-AP

IHC staining of human spleen using 17504-1-AP

IHC staining of human spleen using 17504-1-AP

IHC staining of human lung using 17504-1-AP

IHC staining of human lung using 17504-1-AP

IHC staining of human ovary using 17504-1-AP

IHC staining of human ovary using 17504-1-AP

IHC staining of human heart using 17504-1-AP

IHC staining of human heart using 17504-1-AP

IHC staining of human kidney using 17504-1-AP

IHC staining of human kidney using 17504-1-AP

IHC staining of human kidney using 17504-1-AP

IF Staining of MCF-7 using 17504-1-AP

EIF4A3 Polyclonal antibody

EIF4A3 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

-/ -

Tested Applications

Recommended dilution

Published Applications

Product Information

Background Information

Protocols

Publications

METTL16 promotes liver cancer stem cell self-renewal via controlling ribosome biogenesis and mRNA translation

NEAT1 is essential for metabolic changes that promote breast cancer growth and metastasis.

YTHDF3 facilitates translation and decay of N(6)-methyladenosine-modified RNA.

Hypoxia-enhanced YAP1-EIF4A3 interaction drives circ_0007386 circularization by competing with CRIM1 pre-mRNA linear splicing and promotes non-small cell lung cancer progression

eIF4A3 regulates the TFEB-mediated transcriptional response via GSK3B to control autophagy.

Long noncoding RNA ZFAS1 promoting small nucleolar RNA-mediated 2'-O-methylation via NOP58 recruitment in colorectal cancer.