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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using EIF3L Monoclonal antibody (67719-1-Ig)

    WB analysis using 67719-1-Ig (same clone as 67719-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67719-1-Ig (EIF3L antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67719-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using EIF3L Monoclonal antibody (67719-1-Ig)

    WB analysis using 67719-1-Ig (same clone as 67719-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67719-1-Ig (EIF3L antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67719-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse pancreas tissue using EIF3L Monoclonal antibody (67719-1-Ig)

    IHC staining of mouse pancreas using 67719-1-Ig (same clone as 67719-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue slide using 67719-1-Ig (EIF3L antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67719-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse pancreas tissue using EIF3L Monoclonal antibody (67719-1-Ig)

    IHC staining of mouse pancreas using 67719-1-Ig (same clone as 67719-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue slide using 67719-1-Ig (EIF3L antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67719-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of mouse pancreas tissue using EIF3L Monoclonal antibody (67719-1-Ig)

    IF Staining of mouse pancreas using 67719-1-Ig (same clone as 67719-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed mouse pancreas tissue using EIF3L antibody (67719-1-Ig, Clone: 1B11A10 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67719-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of mouse pancreas tissue using EIF3L Monoclonal antibody (67719-1-Ig)

    IF Staining of mouse pancreas using 67719-1-Ig (same clone as 67719-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed mouse pancreas tissue using EIF3L antibody (67719-1-Ig, Clone: 1B11A10 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67719-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using EIF3L Monoclonal antibody (67719-1-Ig)

    IF Staining of HeLa using 67719-1-Ig (same clone as 67719-1-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using EIF3L antibody (67719-1-Ig, Clone: 1B11A10 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67719-1-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of HeLa cells using EIF3L Monoclonal antibody (67719-1-Ig)

    FC experiment of HeLa using 67719-1-Ig (same clone as 67719-1-PBS)

    1X10^6 HeLa cells were intracellularly stained with 0.4 ug Anti-Human EIF3L (67719-1-Ig, Clone:1B11A10) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Mouse IgG2a Isotype Control (C1.18.4) (65208-1-Ig, Clone: C1.18.4) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer. This data was developed using the same antibody clone with 67719-1-PBS in a different storage buffer formulation.

EIF3L Monoclonal antibody, PBS Only

EIF3L Monoclonal Antibody for WB, IHC, IF/ICC, IF-P, FC (Intra), Indirect ELISA
Cat No. 67719-1-PBS
Clone No.1B11A10

Host / Isotype

Mouse / IgG2a

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IF-P, FC (Intra), Indirect ELISA

Synonyms

1B11A10, EIF3EIP, EIF3S11, EIF3S6IP, Eukaryotic translation initiation factor 3 subunit 6-interacting protein

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration:  100 μg, 1mg/ml
100 μg, 1mg/ml

¥167,000/ 100 μg

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

67719-1-PBS targets EIF3L in WB, IHC, IF/ICC, IF-P, FC (Intra), Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen

CatNo: Ag26680

Product name: Recombinant human EIF3L protein

Source: e coli.-derived, PET30a

Tag: 6*His

Domain: 255-564 aa of BC007510

Sequence: SVAGEYGRHSLYKMLGYFSLVGLLRLHSLLGDYYQAIKVLENIELNKKSMYSRVPECQVTTYYYVGFAYLMMRRYQDAIRVFANILLYIQRTKSMFQRTTYKYEMINKQNEQMHALLAIALTMYPMRIDESIHLQLREKYGDKMLRMQKGDPQVYEELFSYSCPKFLSPVVPNYDNVHPNYHKEPFLQQLKVFSDEVQQQAQLSTIRSFLKLYTTMPVAKLAGFLDLTEQEFRIQLLVFKHKMKNLVWTSGISALDGEFQSASEVDFYIDKDMIHIADTKVARRYGDFFIRQIHKFEELNRTLKKMGQRP

相同性解析による交差性が予測される生物種
Full Name eukaryotic translation initiation factor 3, subunit L
Calculated molecular weight 67 kDa
Observed molecular weight67 kDa
GenBank accession numberBC007510
Gene Symbol EIF3L
Gene ID (NCBI) 51386
RRIDAB_2882907
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein A purification
UNIPROT IDQ9Y262
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.
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