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Validation Data Gallery

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  • Western Blot (WB) analysis of HeLa cells using EIF3J Polyclonal antibody (10439-1-AP)

    WB analysis of HeLa using 10439-1-AP (same clone as 10439-1-PBS)

    HeLa cells were subjected to SDS PAGE followed by western blot with 10439-1-AP (EIF3J antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10439-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HeLa cells using EIF3J Polyclonal antibody (10439-1-AP)

    WB analysis of HeLa using 10439-1-AP (same clone as 10439-1-PBS)

    HeLa cells were subjected to SDS PAGE followed by western blot with 10439-1-AP (EIF3J antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10439-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of MCF-7 cells using EIF3J Polyclonal antibody (10439-1-AP)

    WB analysis of MCF-7 using 10439-1-AP (same clone as 10439-1-PBS)

    MCF7 cells were subjected to SDS PAGE followed by western blot with 10439-1-AP (EIF3J antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10439-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of Jurkat cells using EIF3J Polyclonal antibody (10439-1-AP)

    WB analysis of Jurkat using 10439-1-AP (same clone as 10439-1-PBS)

    Jurkat cells were subjected to SDS PAGE followed by western blot with 10439-1-AP (EIF3J antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10439-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HEK-293 cells using EIF3J Polyclonal antibody (10439-1-AP)

    WB analysis of HEK-293 using 10439-1-AP (same clone as 10439-1-PBS)

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 10439-1-AP (EIF3J antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10439-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of A549 cells using EIF3J Polyclonal antibody (10439-1-AP)

    WB analysis of A549 using 10439-1-AP (same clone as 10439-1-PBS)

    A549 cells were subjected to SDS PAGE followed by western blot with 10439-1-AP (EIF3J antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10439-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HeLa cells using EIF3J Polyclonal antibody (10439-1-AP)

    WB analysis of HeLa using 10439-1-AP (same clone as 10439-1-PBS)

    HeLa cells were subjected to SDS PAGE followed by western blot with 10439-1-AP (EIF3J antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10439-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HeLa cells using EIF3J Polyclonal antibody (10439-1-AP)

    WB analysis of HeLa using 10439-1-AP (same clone as 10439-1-PBS)

    HeLa cells were subjected to SDS PAGE followed by western blot with 10439-1-AP (EIF3J antibody) at dilution of 1:0 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10439-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of K-562 cells using EIF3J Polyclonal antibody (10439-1-AP)

    WB analysis of K-562 using 10439-1-AP (same clone as 10439-1-PBS)

    K-562 cells were subjected to SDS PAGE followed by western blot with 10439-1-AP (EIF3J antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10439-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of SKOV-3 cells using EIF3J Polyclonal antibody (10439-1-AP)

    WB analysis of SKOV-3 using 10439-1-AP (same clone as 10439-1-PBS)

    SKOV-3 cells were subjected to SDS PAGE followed by western blot with 10439-1-AP (EIF3J antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10439-1-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of HeLa cells using EIF3J Polyclonal antibody (10439-1-AP)

    IP experiment of HeLa using 10439-1-AP (same clone as 10439-1-PBS)

    IP result of anti-EIF3J (IP:10439-1-AP, 4ug; Detection:10439-1-AP 1:500) with HeLa cells lysate 880ug. This data was developed using the same antibody clone with 10439-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using EIF3J Polyclonal antibody (10439-1-AP)

    IHC staining of human breast cancer using 10439-1-AP (same clone as 10439-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer using 10439-1-AP (EIF3J antibody) at dilution of 1:100 (under 10x lens). This data was developed using the same antibody clone with 10439-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using EIF3J Polyclonal antibody (10439-1-AP)

    IHC staining of human breast cancer using 10439-1-AP (same clone as 10439-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer using 10439-1-AP (EIF3J antibody) at dilution of 1:10 (under 10x lens). This data was developed using the same antibody clone with 10439-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using EIF3J Polyclonal antibody (10439-1-AP)

    IF Staining of HeLa using 10439-1-AP (same clone as 10439-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using EIF3J antibody (10439-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 10439-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO Validated

EIF3J Polyclonal antibody, PBS Only

EIF3J Polyclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA
Cat No. 10439-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, IP, Indirect ELISA

Synonyms

Eukaryotic translation initiation factor 3 subunit 1, EIF3S1, eIF-3-alpha, eIF3 p35, eIF3 alpha

Formulation:  PBS Only
PBS, Azide, Glycerol
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

10439-1-PBS targets EIF3J in WB, IHC, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag0678

Product name: Recombinant human EIF3J protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-258 aa of BC002719

Sequence: MAAAAAAAGDSDSWDADAFSVEDPVRKVGGGGTAGGDRWEGEDEDEDVKDNWDDDDDEKKEEAEVKPEVKISEKKKIAEKIKEKERQQKKRQEEIKKRLEEPEEPKVLTPEEQLADKLRLKKLQEESDLELAKETFGVNNAVYGIDAMNPSSRDDFTEFGKLLKDKITQYEKSLYYASFLEVLVRDVCISLEIDDLKKITNSLTVLCSEKQKQEKQSKAKKKKKGVVPGGGLKATMKDDLADYGGYDGGYVQDYEDFM

相同性解析による交差性が予測される生物種
Full Name eukaryotic translation initiation factor 3, subunit J
Calculated molecular weight 28 kDa
Observed molecular weight 35 kDa
GenBank accession numberBC002719
Gene Symbol EIF3J
Gene ID (NCBI) 8669
RRIDAB_2097082
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDO75822
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

EIF3 has a key role in binding of initiator methionyl-tRNA and mRNA to the 40S ribosomal subunit to form the 40S initiation complex(PMID:17588516). The eIF3 complex stimulates several steps in the translation initiation pathway, including dissociation of 80S ribosomes into 40S and 60S subunits, binding of a ternary complex (TC) consisting of Met-tRNA , eIF2, and GTP to the small subunit (forming the 43S preinitiation complex) and recruitment of mRNA to the 43 S complex to produce the 48S complex (PMID:11560931) EIF3J was identified as a high copy suppressor of the temperature-sensitive (Ts-) phenotype of the rpg1-1 allele of TIF32/RPG1, encoding the largest subunit of yeast eIF3 (eIF3a) (PMID:10488093).

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