• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0
×

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of HepG2 cells using EIF3G Polyclonal antibody (11165-1-AP)

    WB analysis of HepG2 using 11165-1-AP (same clone as 11165-1-PBS)

    HepG2 cells were subjected to SDS PAGE followed by western blot with 11165-1-AP (EIF3G antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11165-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using EIF3G Polyclonal antibody (11165-1-AP)

    IHC staining of human colon cancer using 11165-1-AP (same clone as 11165-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 11165-1-AP (EIF3G antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11165-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using EIF3G Polyclonal antibody (11165-1-AP)

    IHC staining of human colon cancer using 11165-1-AP (same clone as 11165-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 11165-1-AP (EIF3G antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11165-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse colon tissue using EIF3G Polyclonal antibody (11165-1-AP)

    IHC staining of mouse colon using 11165-1-AP (same clone as 11165-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 11165-1-AP (EIF3G antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11165-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse colon tissue using EIF3G Polyclonal antibody (11165-1-AP)

    IHC staining of mouse colon using 11165-1-AP (same clone as 11165-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 11165-1-AP (EIF3G antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11165-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of rat colon tissue using EIF3G Polyclonal antibody (11165-1-AP)

    IHC staining of rat colon using 11165-1-AP (same clone as 11165-1-PBS)

    Immunohistochemical analysis of paraffin-embedded rat colon tissue slide using 11165-1-AP (EIF3G antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11165-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of rat colon tissue using EIF3G Polyclonal antibody (11165-1-AP)

    IHC staining of rat colon using 11165-1-AP (same clone as 11165-1-PBS)

    Immunohistochemical analysis of paraffin-embedded rat colon tissue slide using 11165-1-AP (EIF3G antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11165-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using EIF3G Polyclonal antibody (11165-1-AP)

    IF Staining of HepG2 using 11165-1-AP (same clone as 11165-1-PBS)

    Immunofluorescent analysis of HepG2 cells using 11165-1-AP (EIF3G antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 11165-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of A549 cells using EIF3G Polyclonal antibody (11165-1-AP)

    IF Staining of A549 using 11165-1-AP (same clone as 11165-1-PBS)

    Immunofluorescent analysis of A549 cells, using EIF3G antibody 11165-1-AP at 1:50 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye). This data was developed using the same antibody clone with 11165-1-PBS in a different storage buffer formulation.

［PR］まずは試したいあなたに。トライアルサイズも特別価格 >>『PRIME BUY抗体特割キャンペーン（4/21～）』

EIF3G Polyclonal antibody, PBS Only

EIF3G Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 11165-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

eIF-3 RNA-binding subunit, eIF3 p44, eIF3 p42, eIF3 delta, eIF 3 RNA binding subunit

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

ご購入について

国内販売は「コスモ・バイオ株式会社」を通じて行っております。お見積り・ご注文はお近くの販売代理店へご連絡ください。

国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

11165-1-PBS targets EIF3G in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen EIF3G fusion protein Ag1653 相同性解析による交差性が予測される生物種
Full Name eukaryotic translation initiation factor 3, subunit G
Calculated molecular weight 36 kDa
Observed molecular weight 44 kDa
GenBank accession numberBC000733
Gene Symbol EIF3G
Gene ID (NCBI) 8666
RRIDAB_2096911
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDO75821
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

Eukaryotic translation initiation factor eIF3, that plays a central role in translation initiation, consists of five core subunits that are present in both the budding yeast and higher eukaryotes. The EIF-3 complex associates with the 40S ribosome and facilitates the recruitment of eIF-1, eIF-1A, eIF-2:GTP:methionyl-tRNAi and eIF-5 to form the 43S preinitiation complex. The complex stimulates mRNA recruitment to the 43S PIC and scanning of the mRNA for AUG recognition. It is also required for disassembly and recycling of post-termination ribosomal complexes and subsequently prevents premature joining of the 40S and 60S ribosomal subunits prior to initiation. EIF3G is a component of the EIF3 complex. [PMID:20679478,20503360]