• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0
×

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

EIF3E Polyclonal antibody

EIF3E Polyclonal Antibody for WB, IP, IF, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IF, IHC, ELISA

Conjugate

Unconjugated

Cat no : 11352-1-AP

Synonyms

eIF 3 p48, eIF3 p46, EIF3 P48, EIF3E, EIF3S6, INT6

Validation Data Gallery

Applications:
  • WB analysis of HeLa using 11352-1-AP

    WB analysis of HeLa using 11352-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 11352-1-AP (EIF3E antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • WB analysis of HepG2 using 11352-1-AP

    WB analysis of HepG2 using 11352-1-AP

    HepG2 cells were subjected to SDS PAGE followed by western blot with 11352-1-AP (EIF3E antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • WB analysis of HEK-293 using 11352-1-AP

    WB analysis of HEK-293 using 11352-1-AP

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 11352-1-AP (EIF3E antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • WB analysis of Jurkat using 11352-1-AP

    WB analysis of Jurkat using 11352-1-AP

    Jurkat cells were subjected to SDS PAGE followed by western blot with 11352-1-AP (EIF3E antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • IP experiment of HepG2 using 11352-1-AP

    IP experiment of HepG2 using 11352-1-AP

    IP result of anti-EIF3E (IP:11352-1-AP, 4ug; Detection:11352-1-AP 1:500) with HepG2 cells lysate 880 ug.

  • IHC staining of human breast cancer using 11352-1-AP

    IHC staining of human breast cancer using 11352-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 11352-1-AP (EIF3E antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with EIF3E antibody included
  • IHC staining of human breast cancer using 11352-1-AP

    IHC staining of human breast cancer using 11352-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 11352-1-AP (EIF3E antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with EIF3E antibody included
  • IF Staining of HepG2 using 11352-1-AP

    IF Staining of HepG2 using 11352-1-AP

    Immunofluorescent analysis of HepG2 cells using 11352-1-AP (EIF3E antibody) at dilution of 1:25 and Rhodamine-Goat anti-Rabbit IgG.

  • IF Staining of HepG2 using 11352-1-AP

    IF Staining of HepG2 using 11352-1-AP

    Immunofluorescent analysis of Ethacrynic acid treated HepG2 cells using 11352-1-AP (EIF3E antibody) at dilution of 1:25 and Rhodamine-Goat anti-Rabbit IgG.

Tested Applications

Positive WB detected inHeLa cells, HEK-293 cells, HepG2 cells, Jurkat cells
Positive IP detected inHepG2 cells
Positive IHC detected inhuman breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells
Planning an IHC experiment? We recommend our IHCeasy EIF3E Ready-To-Use IHC Kit. EIF3E primary antibody included.

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2400
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:10-1:100
Sample-dependent, check data in validation data gallery

Product Information

11352-1-AP targets EIF3E in WB, IP, IF, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen EIF3E fusion protein Ag1856 相同性解析による交差性が予測される生物種
Full Name eukaryotic translation initiation factor 3, subunit E
Calculated molecular weight 48 kDa
Observed molecular weight 48-52 kDa
GenBank accession numberBC000734
Gene symbol EIF3E
Gene ID (NCBI) 3646
RRIDAB_2277716
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for EIF3E antibody 11352-1-APDownload protocol
IHC protocol for EIF3E antibody 11352-1-APDownload protocol
IF protocol for EIF3E antibody 11352-1-APDownload protocol
IP protocol for EIF3E antibody 11352-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols