Validation Data Gallery

Filter: AllWBIPIHCIF/ICC

WB analysis of rat lymph using 10899-1-AP
rat lymph tissue were subjected to SDS PAGE followed by western blot with 10899-1-AP (EIF3E antibody) at dilution of 1:100 incubated at room temperature for 1.5 hours.
WB analysis of HeLa using 10899-1-AP
HeLa cells were subjected to SDS PAGE followed by western blot with 10899-1-AP (EIF3E antibody) at dilution of 1:100 incubated at room temperature for 1.5 hours.
WB analysis of mouse thymus using 10899-1-AP
mouse thymus tissue were subjected to SDS PAGE followed by western blot with 10899-1-AP (EIF3E antibody) at dilution of 1:100 incubated at room temperature for 1.5 hours.
WB analysis of mouse pancreas using 10899-1-AP
mouse pancreas tissue were subjected to SDS PAGE followed by western blot with 10899-1-AP (EIF3E antibody) at dilution of 1:100 incubated at room temperature for 1.5 hours.
WB analysis of Jurkat using 10899-1-AP
Jurkat cells were subjected to SDS PAGE followed by western blot with 10899-1-AP (EIF3E antibody) at dilution of 1:100 incubated at room temperature for 1.5 hours.
IP experiment of Jurkat using 10899-1-AP
IP result of anti-EIF3E (IP:10899-1-AP, 4ug; Detection:10899-1-AP 1:500) with Jurkat cells lysate 2440 ug.
IHC staining of human pancreas cancer using 10899-1-AP
Immunohistochemical analysis of paraffin-embedded human pancreas cancer using 10899-1-AP (EIF3E antibody) at dilution of 1:100 (under 10x lens).
IF Staining of SH-SY5Y using 10899-1-AP
Immunofluorescent analysis of (-20°C Ethanol) fixed SH-SY5Y cells using EIF3E antibody (10899-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).
IF Staining of U2OS using 10899-1-AP
Immunofluorescent analysis of (-20°C Ethanol) fixed U2OS cells using EIF3E antibody (10899-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

EIF3E Polyclonal antibody

EIF3E Polyclonal Antibody for WB, IHC, IP, ELISA

Cat No. 10899-1-AP

Publications(4)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IHC, IF, IP, ELISA

Synonyms

Viral integration site protein INT-6 homolog, INT6, EIF3S6, eIF-3 p48

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

Conjugate: Unconjugated

Unconjugated

CoraLite® Plus 488

Size/Concentration:

-/ -

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Overview

Tested Applications
Product Information
Publications (4)
Protocols

データシート［PDF］を印刷

MSDS

Tested Applications

Positive WB detected in	rat lymph tissue, HeLa cells, mouse thymus tissue, mouse pancreas tissue, Jurkat cells
Positive IP detected in	Jurkat cells
Positive IHC detected in	human pancreas cancer tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:500-1:1000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WB	See 3 publications below
IHC	See 1 publications below
IF	See 1 publications below

Product Information

10899-1-AP targets EIF3E in WB, IHC, IF, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human, pig
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag1132 Product name: Recombinant human EIF3E protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 145-445 aa of BC008419 Sequence: SGAAEYLYFFRVLVPATDRNALSSLWGKLASEILMQNWDAAMEDLTRLKETIDNNSVSSPLQSLQQRTWLIHWSLFVFFNHPKGRDNIIDLFLYQPQYLNAIQTMCPHILRYLTTAVITNKDVRKRRQVLKDLVKVIQQESYTYKDPITEFVECLYVNFDFDGAQKKLRECESVLVNDFFLVACLEDFIENARLFIFETFCRIHQCISINMLADKLNMTPEEAERWIVNLIRNARLDAKIDSKLGHVVMGNNAVSPYQQVIEKTKSLSFRSQMLAMNIEKKLNQNSRSEAPNWATQDSGFY 相同性解析による交差性が予測される生物種
Full Name	eukaryotic translation initiation factor 3, subunit E
Calculated molecular weight	445 aa, 52 kDa
Observed molecular weight	48-52 kDa
GenBank accession number	BC008419
Gene Symbol	EIF3E
Gene ID (NCBI)	3646
RRID	AB_2096898
Conjugate	Unconjugated
Form
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	P60228
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Protocols

Product Specific Protocols
IF protocol for EIF3E antibody 10899-1-AP	Download protocol
IHC protocol for EIF3E antibody 10899-1-AP	Download protocol
IP protocol for EIF3E antibody 10899-1-AP	Download protocol
WB protocol for EIF3E antibody 10899-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human	WB,IF	Aging Cell Alzheimer's disease associated AKAP9 I2558M mutation alters posttranslational modification and interactome of tau and cellular functions in CRISPR-edited human neuronal cells. Authors - Yang You View Article
human	IHC	Cancer Genomics Proteomics Biomarker Expression Profiling in Cervix Carcinoma Biopsies Unravels WT1 as a Target of Artesunate Authors - MOHAMED E. M. SAEED View Article
human	WB	Cancer Lett N6-Methyladenosine enhances the translation of ENO1 to promote the progression of bladder cancer by inhibiting PCNA ubiquitination Authors - Chengquan Shen View Article
pig	WB	J Virol VP3 protein of Senecavirus A promotes viral IRES-driven translation and attenuates innate immunity by specifically relocalizing hnRNPA2B1 Authors - Lu Li View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Validation Data Gallery

WB analysis of rat lymph using 10899-1-AP

WB analysis of HeLa using 10899-1-AP

WB analysis of mouse thymus using 10899-1-AP

WB analysis of mouse pancreas using 10899-1-AP

WB analysis of Jurkat using 10899-1-AP

IP experiment of Jurkat using 10899-1-AP

IHC staining of human pancreas cancer using 10899-1-AP

IF Staining of SH-SY5Y using 10899-1-AP

IF Staining of U2OS using 10899-1-AP

EIF3E Polyclonal antibody

EIF3E Polyclonal Antibody for WB, IHC, IP, ELISA

-/ -

Tested Applications

Recommended dilution

Published Applications

Product Information

Protocols

Publications

Alzheimer's disease associated AKAP9 I2558M mutation alters posttranslational modification and interactome of tau and cellular functions in CRISPR-edited human neuronal cells.

Biomarker Expression Profiling in Cervix Carcinoma Biopsies Unravels WT1 as a Target of Artesunate

N6-Methyladenosine enhances the translation of ENO1 to promote the progression of bladder cancer by inhibiting PCNA ubiquitination

VP3 protein of Senecavirus A promotes viral IRES-driven translation and attenuates innate immunity by specifically relocalizing hnRNPA2B1