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Species Reactivity
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Conjugate
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Conjugate
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Conjugate
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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

EIF3C Polyclonal antibody

EIF3C Polyclonal Antibody for IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, ELISA

Conjugate

Unconjugated

Cat no : 12733-1-AP

Synonyms

eIF3 p110, EIF3C, EIF3CL, EIF3S8

Validation Data Gallery

Applications:
  • WB analysis of K-562 using 12733-1-AP

    WB analysis of K-562 using 12733-1-AP

    K-562 cells were subjected to SDS PAGE followed by western blot with 12733-1-AP (EIF3C antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • WB analysis of MCF-7 using 12733-1-AP

    WB analysis of MCF-7 using 12733-1-AP

    MCF7 cells were subjected to SDS PAGE followed by western blot with 12733-1-AP (EIF3C antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • IP experiment of K-562 using 12733-1-AP

    IP experiment of K-562 using 12733-1-AP

    IP result of anti-EIF3C (IP:12733-1-AP, 4ug; Detection:12733-1-AP 1:500) with K-562 cells lysate 3200ug.

  • IHC staining of human colon cancer using 12733-1-AP

    IHC staining of human colon cancer using 12733-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 12733-1-AP (EIF3C antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with EIF3C antibody included
  • IHC staining of human colon cancer using 12733-1-AP

    IHC staining of human colon cancer using 12733-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 12733-1-AP (EIF3C antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with EIF3C antibody included

Tested Applications

Positive WB detected inK-562 cells, MCF-7 cells
Positive IP detected inK-562 cells
Planning an IHC experiment? We recommend our IHCeasy EIF3C Ready-To-Use IHC Kit. EIF3C primary antibody included.

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Sample-dependent, check data in validation data gallery

Published Applications

WBSee 1 publications below

Product Information

12733-1-AP targets EIF3C in WB, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen EIF3C fusion protein Ag3438 相同性解析による交差性が予測される生物種
Full Name eukaryotic translation initiation factor 3, subunit C
Calculated molecular weight 913 aa, 110 kDa
Observed molecular weight 110 kDa
GenBank accession numberBC001571
Gene symbol EIF3C
Gene ID (NCBI) 8663
RRIDAB_2096747
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for EIF3C antibody 12733-1-APDownload protocol
IHC protocol for EIF3C antibody 12733-1-APDownload protocol
IP protocol for EIF3C antibody 12733-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Cell Death Differ

Loss of SMURF2 expression enhances RACK1 stability and promotes ovarian cancer progression

Authors - Yanan Pi
View Article