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Validation Data Gallery

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  • Western Blot (WB) analysis of mouse thymus tissue using EIF2S3 Polyclonal antibody (11162-1-AP)

    WB analysis of mouse thymus using 11162-1-AP (same clone as 11162-1-PBS)

    mouse thymus tissue were subjected to SDS PAGE followed by western blot with 11162-1-AP (EIF2S3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11162-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HeLa cells using EIF2S3 Polyclonal antibody (11162-1-AP)

    WB analysis of HeLa using 11162-1-AP (same clone as 11162-1-PBS)

    HeLa cells were subjected to SDS PAGE followed by western blot with 11162-1-AP (EIF2S3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11162-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of mouse thymus tissue using EIF2S3 Polyclonal antibody (11162-1-AP)

    WB analysis of mouse thymus using 11162-1-AP (same clone as 11162-1-PBS)

    mouse thymus tissue were subjected to SDS PAGE followed by western blot with 11162-1-AP (EIF2S3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11162-1-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of HeLa cells using EIF2S3 Polyclonal antibody (11162-1-AP)

    IP experiment of HeLa using 11162-1-AP (same clone as 11162-1-PBS)

    IP result of anti-EIF2S3 (IP:11162-1-AP, 4ug; Detection:11162-1-AP 1:500) with HeLa cells lysate 2800ug. This data was developed using the same antibody clone with 11162-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using EIF2S3 Polyclonal antibody (11162-1-AP)

    IHC staining of human lung cancer using 11162-1-AP (same clone as 11162-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 11162-1-AP (EIF2S3 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11162-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using EIF2S3 Polyclonal antibody (11162-1-AP)

    IHC staining of human lung cancer using 11162-1-AP (same clone as 11162-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 11162-1-AP (EIF2S3 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11162-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using EIF2S3 Polyclonal antibody (11162-1-AP)

    IHC staining of human lung cancer using 11162-1-AP (same clone as 11162-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lung cancer using 11162-1-AP (EIF2S3 antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 11162-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using EIF2S3 Polyclonal antibody (11162-1-AP)

    IHC staining of human lung cancer using 11162-1-AP (same clone as 11162-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lung cancer using 11162-1-AP (EIF2S3 antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 11162-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using EIF2S3 Polyclonal antibody (11162-1-AP)

    IHC staining of human lung cancer using 11162-1-AP (same clone as 11162-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lung cancer using 11162-1-AP (EIF2S3 antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 11162-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using EIF2S3 Polyclonal antibody (11162-1-AP)

    IHC staining of human lung cancer using 11162-1-AP (same clone as 11162-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lung cancer using 11162-1-AP (EIF2S3 antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 11162-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using EIF2S3 Polyclonal antibody (11162-1-AP)

    IHC staining of human tonsillitis using 11162-1-AP (same clone as 11162-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human tonsillitis using 11162-1-AP (EIF2S3 antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 11162-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using EIF2S3 Polyclonal antibody (11162-1-AP)

    IHC staining of human tonsillitis using 11162-1-AP (same clone as 11162-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human tonsillitis using 11162-1-AP (EIF2S3 antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 11162-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using EIF2S3 Polyclonal antibody (11162-1-AP)

    IF Staining of HeLa using 11162-1-AP (same clone as 11162-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using EIF2S3 antibody (11162-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 11162-1-PBS in a different storage buffer formulation.

EIF2S3 Polyclonal antibody, PBS Only

EIF2S3 Polyclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA
Cat No. 11162-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, IP, Indirect ELISA

Synonyms

EIF2G, EIF2, eIF 2gX, eIF 2gA, eIF 2 gamma X

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

11162-1-PBS targets EIF2S3 in WB, IHC, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag1650

Product name: Recombinant human EIF2S3 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 173-472 aa of BC019906

Sequence: HLAAIEIMKLKHILILQNKIDLVKESQAKEQYEQILAFVQGTVAEGAPIIPISAQLKYNIEVVCEYIVKKIPVPPRDFTSEPRLIVIRSFDVNKPGCEVDDLKGGVAGGSILKGVLKVGQEIEVRPGIVSKDSEGKLMCKPIFSKIVSLFAEHNDLQYAAPGGLIGVGTKIDPTLCRADRMVGQVLGAVGALPEIFTELEISYFLLRRLLGVRTEGDKKAAKVQKLSKNEVLMVNIGSLSTGGRVSAVKADLGKIVLTNPVCTEVGEKIALSRRVEKHWRLIGWGQIRRGVTIKPTVDDD

相同性解析による交差性が予測される生物種
Full Name eukaryotic translation initiation factor 2, subunit 3 gamma, 52kDa
Calculated molecular weight 52 kDa
Observed molecular weight 52 kDa
GenBank accession numberBC019906
Gene Symbol EIF2S3
Gene ID (NCBI) 1968
RRIDAB_2246331
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDP41091
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

Interacts with GTP and initiator methionyl-tRNA, translation initiation factor eIF2 forms a ternary complex that binds the 40S ribosome and then scans an mRNA to select the AUG start codon for protein synthesis[PMID: 9736774]. Junction of the 60S ribosomal subunit to form the 80S initiation complex is preceded by hydrolysis of the GTP bound to eIF-2 and release of an eIF-2-GDP binary complex. In order for eIF-2 to recycle and catalyze another round of initiation, the GDP bound to eIF-2 must exchange with GTP by way of a reaction catalyzed by eIF-2B. EIF2S3 is the core subunit of the heterotrimeric eIF2 complex.[PMID:23063529]

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