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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using EIF2B5 Polyclonal antibody (29935-1-AP)

    WB analysis using 29935-1-AP (same clone as 29935-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 29935-1-AP (EIF2B5 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 29935-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using EIF2B5 Polyclonal antibody (29935-1-AP)

    IHC staining of human colon cancer using 29935-1-AP (same clone as 29935-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 29935-1-AP (EIF2B5 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 29935-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using EIF2B5 Polyclonal antibody (29935-1-AP)

    IHC staining of human colon cancer using 29935-1-AP (same clone as 29935-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 29935-1-AP (EIF2B5 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 29935-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of MCF-7 cells using EIF2B5 Polyclonal antibody (29935-1-AP)

    IF Staining of MCF-7 using 29935-1-AP (same clone as 29935-1-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed MCF-7 cells using EIF2B5 antibody (29935-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 29935-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of MCF-7 cells using EIF2B5 Polyclonal antibody (29935-1-AP)

    IF Staining of MCF-7 using 29935-1-AP (same clone as 29935-1-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed MCF-7 cells using EIF2B5 antibody (29935-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 29935-1-PBS in a different storage buffer formulation.

EIF2B5 Polyclonal antibody, PBS Only

EIF2B5 Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 29935-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

Formulation:  PBS Only
PBS, Azide, Glycerol
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

29935-1-PBS targets EIF2B5 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag32175

Product name: Recombinant human EIF2B5 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 455-711 aa of NM_003907.2

Sequence: DAEEDEDDGEFSDDSGADQEKDKVKMKGYNPAEVGAAGKGYLWKAAGMNMEEEEELQQNLWGLKINMEEESESESEQSMDSEEPDSRGGSPQMDDIKVFQNEVLGTLQRGKEENISCDNLVLEINSLKYAYNISLKEVMQVLSHVVLEFPLQQMDSPLDSSRYCALLLPLLKAWSPVFRNYIKRAADHLEALAAIEDFFLEHEALGISMAKVLMAFYQLEILAEETILSWFSQRDTTDKGQQLRKNQQLQRFIQWLK

相同性解析による交差性が予測される生物種
Full Name eukaryotic translation initiation factor 2B, subunit 5 epsilon, 82kDa
Calculated molecular weight80 kDa
Observed molecular weight65-80 kDa
GenBank accession numberNM_003907.2
Gene Symbol EIF2B5
Gene ID (NCBI) 8893
RRIDAB_2923623
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ13144
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

EIF2B5 encodes translation initiation factor eIF-2B subunit epsilon protein (PMID 8688466). EIF2B5 mutations compromise the induction of GFAP-expressing astrocytes in VWM (vanishing white matter) leukodystrophy (PMID:15723074, PMID:15776425). The upregulation of EIF2B5 is associated with cancer such as human pulmonary adenocarcinoma cell and breast cancer (PMID:24366584, PMID:24979338). EIF2B5 also has 65kDa truncated protein isoform (PMID:28961236).

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