Validation Data Gallery
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Tested Applications
| Positive WB detected in | HeLa cells, PC-3 cells |
| Positive IHC detected in | mouse colon tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HeLa cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:20-1:200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 1 publications below |
| IF | See 1 publications below |
Product Information
11296-2-AP targets EIF2B3 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Cited Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag1809 Product name: Recombinant human EIF2B3 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 15-232 aa of BC018728 Sequence: GSILQKHPRIRFHTGLVDAHLYCLKKYIVDFLMENGSITSIRSELIPYLVRKQFSSASSQQGQEEKEEDLKKKELKSLDIYSFIKEANTLNLAPYDACWNACRGDRWEDLSRSQVRCYVHIMKEGLCSRVSTLGLYMEANRQVPKLLSALCPEEPPVHSSAQIVSKHLVGVDSLIGPETQIGEKSSIKRSVIGSSCLIKDRVTITNCLLMNSVTVEEG 相同性解析による交差性が予測される生物種 |
| Full Name | eukaryotic translation initiation factor 2B, subunit 3 gamma, 58kDa |
| Calculated molecular weight | 44 kDa, 50 kDa |
| Observed molecular weight | 50 kDa |
| GenBank accession number | BC018728 |
| Gene Symbol | EIF2B3 |
| Gene ID (NCBI) | 8891 |
| RRID | AB_2096142 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9NR50 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for EIF2B3 antibody 11296-2-AP | Download protocol |
| IHC protocol for EIF2B3 antibody 11296-2-AP | Download protocol |
| WB protocol for EIF2B3 antibody 11296-2-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Elife The small molecule ISRIB rescues the stability and activity of Vanishing White Matter Disease eIF2B mutant complexes. | ||
Mol Biol Cell Cellular eIF2B subunit localization: implications for the integrated stress response and its control by small molecule drugs. |
