Validation Data Gallery
Tested Applications
Recommended dilution
| Application | Dilution |
|---|---|
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Product Information
27944-1-PBS targets EIF2AK4 in WB, IHC, Indirect ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag27617 Product name: Recombinant human EIF2AK4 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 875-1080 aa of BC009350 Sequence: AFSADSKQDDQTGDLIKSDPSGHLTGMVGTALYVSPEVQGSTKSAYNQKVDLFSLGIIFFEMSYHPMVTASERIFVLNQLRDPTSPKFPEDFDDGEHAKQKSVISWLLNHDPAKRPTATELLKSELLPPPQMEESELHEVLHHTLTNVDGKAYRTMMAQIFSQRISPAIDYTYDSDILKGNFSIRTAKMQQHVCETIIRIFKRHGA 相同性解析による交差性が予測される生物種 |
| Full Name | eukaryotic translation initiation factor 2 alpha kinase 4 |
| Calculated molecular weight | 187 kDa |
| Observed molecular weight | ~200 kDa |
| GenBank accession number | BC009350 |
| Gene Symbol | EIF2AK4 |
| Gene ID (NCBI) | 440275 |
| RRID | AB_3669630 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9P2K8 |
| Storage Buffer | PBS only{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -80°C. |
Background Information
EIF2AK4 (eukaryotic translation initiation factor 2 α kinase 4), formerly known as GCN2, a member of serine/threonine kinases, is comprised of an N-terminal RWD domain (20-137) involved in protein-protein interactions, pseudokinase (276-539) and kinase domains (585-1016) and C-terminal domain (1533-1649) where binding to ribosomes and recognition of uncharged tRNAs occur. It downregulates protein synthesis in response to a variety of cellular stress such as hypoxia, viral infection, and amino acid deprivation (PMID: 38776952, 26300654). Mutant EIF2AK4 could promote proliferation of small pulmonary arteries by crosstalk with mammalian targets of the rapamycin signaling pathway (PMID: 24135949).