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Target

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Species Reactivity

Species Reactivity

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  • Featured Product
  • KD/KO Validated

EIF1AD Polyclonal antibody

EIF1AD Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF, ELISA

Conjugate

Unconjugated

Cat no : 20528-1-AP

Synonyms

EIF1AD, Haponin

Validation Data Gallery

Applications:
  • WB analysis of K-562 using 20528-1-AP

    WB analysis of K-562 using 20528-1-AP

    K-562 cells were subjected to SDS PAGE followed by western blot with 20528-1-AP (EIF1AD antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • WB analysis of HEK-293 using 20528-1-AP

    WB analysis of HEK-293 using 20528-1-AP

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 20528-1-AP (EIF1AD antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.

  • WB analysis of HL-60 using 20528-1-AP

    WB analysis of HL-60 using 20528-1-AP

    HL-60 cells were subjected to SDS PAGE followed by western blot with 20528-1-AP (EIF1AD antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.

  • WB analysis of A549 using 20528-1-AP

    WB analysis of A549 using 20528-1-AP

    A549 cells were subjected to SDS PAGE followed by western blot with 20528-1-AP (EIF1AD antibody) at dilution of 1:100 incubated at room temperature for 1.5 hours.

  • WB analysis of MCF-7 using 20528-1-AP

    WB analysis of MCF-7 using 20528-1-AP

    MCF7 cells were subjected to SDS PAGE followed by western blot with 20528-1-AP (EIF1AD antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

Tested Applications

Positive WB detected inK-562 cells, A549 cells, HEK-293 cells, HL-60 cells, MCF-7 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Sample-dependent, check data in validation data gallery

Product Information

The immunogen of 20528-1-AP is EIF1AD Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse, rat
Cited Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen EIF1AD fusion protein Ag14390 相同性解析による交差性が予測される生物種
Full Name eukaryotic translation initiation factor 1A domain containing
Calculated molecular weight 165 aa, 19 kDa
Observed molecular weight 27 kDa
GenBank accession numberBC005131
Gene symbol EIF1AD
Gene ID (NCBI) 84285
RRIDAB_10693533
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for EIF1AD antibody 20528-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB,IF

Nature

Structural basis for the final steps of human 40S ribosome maturation.

Authors - Michael Ameismeier
  • KD Validated
View Article
humanWB

Elife

The final step of 40S ribosomal subunit maturation is controlled by a dual key lock.

Authors - Laura Plassart
View Article
humanWB

Elife

Processing of the ribosomal ubiquitin-like fusion protein FUBI-eS30/FAU is required for 40S maturation and depends on USP36.

Authors - Jasmin van den Heuvel
View Article
humanWB

Life Sci Alliance

CRISPRi screening reveals regulators of tau pathology shared between exosomal and vesicle-free tau

Authors - Juan Carlos Polanco
View Article