Validation Data Gallery

Filter: AllWB

WB analysis of K-562 using 20528-1-AP
K-562 cells were subjected to SDS PAGE followed by western blot with 20528-1-AP (EIF1AD antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of A549 using 20528-1-AP
A549 cells were subjected to SDS PAGE followed by western blot with 20528-1-AP (EIF1AD antibody) at dilution of 1:100 incubated at room temperature for 1.5 hours.
WB analysis of HEK-293 using 20528-1-AP
HEK-293 cells were subjected to SDS PAGE followed by western blot with 20528-1-AP (EIF1AD antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.
WB analysis of HL-60 using 20528-1-AP
HL-60 cells were subjected to SDS PAGE followed by western blot with 20528-1-AP (EIF1AD antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.
WB analysis of MCF-7 using 20528-1-AP
MCF7 cells were subjected to SDS PAGE followed by western blot with 20528-1-AP (EIF1AD antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

Featured Product
KD/KO Validated

EIF1AD Polyclonal antibody

EIF1AD Polyclonal Antibody for WB, ELISA

Cat No. 20528-1-AP

Publications(4)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF, ELISA

Synonyms

EIF1AD, Haponin

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

Conjugate: Unconjugated

Unconjugated

Size/Concentration:

-/ -

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Overview

Tested Applications
Product Information
Publications (4)
Protocols

データシート［PDF］を印刷

MSDS

Tested Applications

Positive WB detected in

K-562 cells, A549 cells, HEK-293 cells, HL-60 cells, MCF-7 cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:500-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 1 publications below
WB	See 4 publications below
IF	See 1 publications below

Product Information

20528-1-AP targets EIF1AD in WB, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag14390 Product name: Recombinant human EIF1AD protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-165 aa of BC005131 Sequence: MSQATKRKHVVKEVLGEHIVPSNQQQIVRVLRTPGNNLHEVETAQGQRFLVSMPSKYRKNIWIKRGDFLIVDPIEEGEKVKAEISFVLCKDHVRSLQKEGFWPEAFSEVAEKHNNRNRQTQPELPAEPQLSGEESSSEDDSDLFVNTNRRQYHESEEESEEEEAA 相同性解析による交差性が予測される生物種
Full Name	eukaryotic translation initiation factor 1A domain containing
Calculated molecular weight	165 aa, 19 kDa
Observed molecular weight	27 kDa
GenBank accession number	BC005131
Gene Symbol	EIF1AD
Gene ID (NCBI)	84285
RRID	AB_10693533
Conjugate	Unconjugated
Form
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	Q8N9N8
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Protocols

Product Specific Protocols
WB protocol for EIF1AD antibody 20528-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human	WB,IF	Nature Structural basis for the final steps of human 40S ribosome maturation. Authors - Michael Ameismeier KD Validated View Article
human	WB	Elife The final step of 40S ribosomal subunit maturation is controlled by a dual key lock. Authors - Laura Plassart View Article
human	WB	Elife Processing of the ribosomal ubiquitin-like fusion protein FUBI-eS30/FAU is required for 40S maturation and depends on USP36. Authors - Jasmin van den Heuvel View Article
human	WB	Life Sci Alliance CRISPRi screening reveals regulators of tau pathology shared between exosomal and vesicle-free tau Authors - Juan Carlos Polanco View Article

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Validation Data Gallery

WB analysis of K-562 using 20528-1-AP

WB analysis of A549 using 20528-1-AP

WB analysis of HEK-293 using 20528-1-AP

WB analysis of HL-60 using 20528-1-AP

WB analysis of MCF-7 using 20528-1-AP