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Validation Data Gallery

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  • Western Blot (WB) analysis of L02 cells using EIF1, EIF1B Polyclonal antibody (15887-1-AP)

    WB analysis of L02 using 15887-1-AP (same clone as 15887-1-PBS)

    L02 cells were subjected to SDS PAGE followed by western blot with 15887-1-AP (EIF1B antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 15887-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HeLa cells using EIF1, EIF1B Polyclonal antibody (15887-1-AP)

    WB analysis of HeLa using 15887-1-AP (same clone as 15887-1-PBS)

    HeLa cells were subjected to SDS PAGE followed by western blot with 15887-1-AP (EIF1B Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 15887-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HepG2 cells using EIF1, EIF1B Polyclonal antibody (15887-1-AP)

    WB analysis of HepG2 using 15887-1-AP (same clone as 15887-1-PBS)

    HepG2 cells were subjected to SDS PAGE followed by western blot with 15887-1-AP (EIF1B antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 15887-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human kidney tissue using EIF1, EIF1B Polyclonal antibody (15887-1-AP)

    IHC staining of human kidney using 15887-1-AP (same clone as 15887-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 15887-1-AP (EIF1B Antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 15887-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human kidney tissue using EIF1, EIF1B Polyclonal antibody (15887-1-AP)

    IHC staining of human kidney using 15887-1-AP (same clone as 15887-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 15887-1-AP (EIF1B Antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 15887-1-PBS in a different storage buffer formulation.

EIF1, EIF1B Polyclonal antibody, PBS Only

EIF1, EIF1B Polyclonal Antibody for WB, IHC, Indirect ELISA
Cat No. 15887-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, Indirect ELISA

Synonyms

Formulation:  PBS Only
PBS, Azide, Glycerol
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

15887-1-PBS targets EIF1, EIF1B in WB, IHC, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag8673

Product name: Recombinant human EIF1B protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-113 aa of BC006996

Sequence: MSTIQNLQSFDPFADATKGDDLLPAGTEDYIHIRIQQRNGRKTLTTVQGIADDYDKKKLVKAFKKKFACNGTVIEHPEYGEVIQLQGDQRKNICQFLLEVGIVKEEQLKVHGF

相同性解析による交差性が予測される生物種
Full Name eukaryotic translation initiation factor 1B
Calculated molecular weight 113 aa, 13 kDa
Observed molecular weight14 kDa
GenBank accession numberBC006996
Gene Symbol EIF1B
Gene ID (NCBI) 10289
RRIDAB_2878196
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDO60739
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.
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