Validation Data Gallery
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Tested Applications
| Positive WB detected in | HEK-293 cells, HeLa cells |
| Positive IF/ICC detected in | A431 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:10000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:1500-1:6000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 8 publications below |
Product Information
66689-1-Ig targets EHMT2 in WB, IF/ICC, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human, mouse |
| Host / Isotype | Mouse / IgG2a |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag27477 Product name: Recombinant human EHMT2 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 652-1001 aa of BC018718 Sequence: ESYHDCVLLFLSRGANPELRNKEGDTAWDLTPERSDVWFALQLNRKLRLGVGNRAIRTEKIICRDVARGYENVPIPCVNGVDGEPCPEDYKYISENCETSTMNIDRNITHLQHCTCVDDCSSSNCLCGQLSIRCWYDKDGRLLQEFNKIEPPLIFECNQACSCWRNCKNRVVQSGIKVRLQLYRTAKMGWGVRALQTIPQGTFICEYVGELISDAEADVREDDSYLFDLDNKDGEVYCIDARYYGNISRFINHLCDPNIIPVRVFMLHQDLRFPRIAFFSSRDIRTGEELGFDYGDRFWDIKSKYFTCQCGSEKCKHSAEAIALEQSRLARLDPHPELLPELGSLPPVNT 相同性解析による交差性が予測される生物種 |
| Full Name | euchromatic histone-lysine N-methyltransferase 2 |
| Calculated molecular weight | 1210 aa, 132 kDa |
| Observed molecular weight | 150 kDa and 170 kDa |
| GenBank accession number | BC018718 |
| Gene Symbol | EHMT2 |
| Gene ID (NCBI) | 10919 |
| RRID | AB_2882043 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | Q96KQ7 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for EHMT2 antibody 66689-1-Ig | Download protocol |
| WB protocol for EHMT2 antibody 66689-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Cell Death Differ Lysine methylation of PPP1CA by the methyltransferase SUV39H2 disrupts TFEB-dependent autophagy and promotes intervertebral disc degeneration | ||
Nat Commun PCGF6 controls neuroectoderm specification of human pluripotent stem cells by activating SOX2 expression. | ||
Am J Cancer Res HSF2 regulates aerobic glycolysis by suppression of FBP1 in hepatocellular carcinoma. | ||
iScience Linkage of methionine addiction, histone lysine hypermethylation, and malignancy. | ||
Front Cell Dev Biol Emerin interacts with histone methyltransferases to regulate repressive chromatin at the nuclear periphery |
