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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

  • Featured Product
  • KD/KO Validated

EHMT2 Polyclonal antibody

EHMT2 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, mouse and More (1)

Applications

WB, IP, IHC, ELISA

Conjugate

Unconjugated

Cat no : 29303-1-AP

Synonyms

BAT8, C6orf30, DKFZp686H08213, EHMT2, FLJ35547, G9A, H3 K9 HMTase 3, HLA B associated transcript 8, KMT1C, Lysine N methyltransferase 1C, NG36, NG36/G9a, Protein G9a

Validation Data Gallery

Applications:
  • WB analysis using 29303-1-AP

    WB analysis using 29303-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 29303-1-AP (EHMT2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • IHC staining of human lung cancer using 29303-1-AP

    IHC staining of human lung cancer using 29303-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 29303-1-AP (EHMT2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human lung cancer using 29303-1-AP

    IHC staining of human lung cancer using 29303-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 29303-1-AP (EHMT2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human urothelial carcinoma using 29303-1-AP

    IHC staining of human urothelial carcinoma using 29303-1-AP

    Immunohistochemical analysis of paraffin-embedded human urothelial carcinoma tissue slide using 29303-1-AP (EHMT2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Tested Applications

Positive WB detected inHepG2 cells, NIH/3T3 cells
Positive IHC detected inhuman lung cancer tissue, human urothelial carcinoma tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

29303-1-AP targets EHMT2 in WB, IP, IHC, ELISA applications and shows reactivity with Human, mouse samples.

Tested Reactivity Human, mouse
Cited Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen EHMT2 fusion protein Ag29160 相同性解析による交差性が予測される生物種
Full Name euchromatic histone-lysine N-methyltransferase 2
Calculated molecular weight 1210 aa, 132 kDa
Observed molecular weight160-180 kDa
GenBank accession numberBC018718
Gene symbol EHMT2
Gene ID (NCBI) 10919
RRIDAB_2918275
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for EHMT2 antibody 29303-1-APDownload protocol
IHC protocol for EHMT2 antibody 29303-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle

Nucleic Acids Res

ATM, KAP1 and the Epstein-Barr virus polymerase processivity factor direct traffic at the intersection of transcription and replication

Authors - Huanzhou Xu
View Article
humanWB,IP

Oncogenesis

Calreticulin enhances gastric cancer metastasis by dimethylating H3K9 in the E-cadherin promoter region mediating by G9a.

Authors - Lina Wang
  • KD Validated
View Article
humanIHC

Mol Biotechnol

Inhibition of the Tumor Suppressor Gene SPINK5 via EHMT2 Induces the Oral Squamous Cell Carcinoma Development

Authors - Suzhen Sun
  • KD Validated
View Article