Validation Data Gallery
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Tested Applications
| Positive WB detected in | HepG2 cells, NIH/3T3 cells |
| Positive IHC detected in | human lung cancer tissue, human urothelial carcinoma tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:6000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 2 publications below |
| WB | See 2 publications below |
| IHC | See 2 publications below |
| IP | See 1 publications below |
Product Information
29303-1-AP targets EHMT2 in WB, IHC, IP, ELISA applications and shows reactivity with Human, mouse samples.
| Tested Reactivity | Human, mouse |
| Cited Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag29160 Product name: Recombinant human EHMT2 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-265 aa of BC018718 Sequence: MSDDVHSLGKVTSDLAKRRKLNSGGGLSEELGSARRSGEVTLTKGDPGSLEEWETVVGDDFSLYYDSYSVDERVDSDSKSEVEALTEQLSEEEEEEEEEEEEEEEEEEEEEEEEDEESGNQSDRSGSSGRRKAKKKWRKDSPWVKPSRKRRKREPPRAKEPRGVNGVGSSGPSEYMEVPLGSLELPSEGTLSPNHAGVSNDTSSLETERGFEELPLCSCRMEAPKIDRISERAGHKCMATESVDGELSGCNAAILKRETMRPSSR 相同性解析による交差性が予測される生物種 |
| Full Name | euchromatic histone-lysine N-methyltransferase 2 |
| Calculated molecular weight | 1210 aa, 132 kDa |
| Observed molecular weight | 160-180 kDa |
| GenBank accession number | BC018718 |
| Gene Symbol | EHMT2 |
| Gene ID (NCBI) | 10919 |
| RRID | AB_2918275 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q96KQ7 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for EHMT2 antibody 29303-1-AP | Download protocol |
| WB protocol for EHMT2 antibody 29303-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Nucleic Acids Res ATM, KAP1 and the Epstein-Barr virus polymerase processivity factor direct traffic at the intersection of transcription and replication | ||
Oncogenesis Calreticulin enhances gastric cancer metastasis by dimethylating H3K9 in the E-cadherin promoter region mediating by G9a.
| ||
Mol Biotechnol Inhibition of the Tumor Suppressor Gene SPINK5 via EHMT2 Induces the Oral Squamous Cell Carcinoma Development
| ||
Mol Carcinog A novel high-risk model identified by epithelial-mesenchymal transition predicts prognosis and radioresistance in rectal cancer |
