Validation Data Gallery
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Tested Applications
| Positive WB detected in | LNCaP cells, A431 cells |
| Positive IHC detected in | human ovary cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 2 publications below |
| WB | See 3 publications below |
| ChIP | See 4 publications below |
Product Information
27195-1-AP targets EHF in WB, IHC, ChIP, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag25885 Product name: Recombinant human EHF protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-199 aa of BC038995 Sequence: MILEGGGVMNLNPGNNLLHQPPAWTDSYSTCNVSSGFFGGQWHEIHPQYWTKYQVWEWLQHLLDTNQLDANCIPFQEFDINGEHLCSMSLQEFTRAAGTAGQLLYSNLQHLKWNGQCSSDLFQSTHNVIVKTEQTEPSIMNTWKDENYLYDTNYGSTVDLLDSKTFCRAQISMTTTSHLPVAESPDMKKEQDPPAKCHT 相同性解析による交差性が予測される生物種 |
| Full Name | ets homologous factor |
| Calculated molecular weight | 35 kDa |
| Observed molecular weight | 35 kDa |
| GenBank accession number | BC038995 |
| Gene Symbol | EHF |
| Gene ID (NCBI) | 26298 |
| RRID | AB_2880796 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9NZC4 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for EHF antibody 27195-1-AP | Download protocol |
| WB protocol for EHF antibody 27195-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Oncogenesis Aberrant super-enhancer landscape reveals core transcriptional regulatory circuitry in lung adenocarcinoma.
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Am J Cancer Res Ese-3 contributes to colon cancer progression by downregulating EHD2 and transactivating INPP4B.
| ||
Gene ETS homologous factor, controlled by lysine-specific demethylase 5B, suppresses clear cell renal cell carcinoma by inducing Filamin-B | ||
J Clin Endocrinol Metab BRAF-induced EHF expression affects TERT in aggressive papillary thyroid cancer | ||
Endocrinology NEAT1_2/RRAD/EHF positive feedback loop facilitates aerobic glycolysis in papillary thyroid cancer cells |
