Validation Data Gallery

Filter: AllWBIHC

WB analysis using 25320-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 25320-1-AP (EHD3 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.
WB analysis of human peripheral blood platelets using 25320-1-AP
human peripheral blood platelets were subjected to SDS PAGE followed by western blot with 25320-1-AP (EHD3 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.
WB analysis of rat brain using 25320-1-AP
rat brain tissue were subjected to SDS PAGE followed by western blot with 25320-1-AP (EHD3 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.
WB analysis of mouse kidney using 25320-1-AP
mouse kidney tissue were subjected to SDS PAGE followed by western blot with 25320-1-AP (EHD3 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.
IHC staining of mouse lung using 25320-1-AP
Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 25320-1-AP (EHD3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse lung using 25320-1-AP
Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 25320-1-AP (EHD3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse kidney using 25320-1-AP
Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 25320-1-AP (EHD3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse kidney using 25320-1-AP
Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 25320-1-AP (EHD3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human brain using 25320-1-AP
Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 25320-1-AP (EHD3 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human brain using 25320-1-AP
Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 25320-1-AP (EHD3 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse brain using 25320-1-AP
Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 25320-1-AP (EHD3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse brain using 25320-1-AP
Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 25320-1-AP (EHD3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Featured Product
KD/KO Validated

EHD3 Polyclonal antibody

EHD3 Polyclonal Antibody for WB, IHC, ELISA

Cat No. 25320-1-AP

Publications(6)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IF, IHC, ELISA

Synonyms

EH domain containing 3, EH domain containing protein 3, EHD2, EHD3, PAST homolog 3, PAST3

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

Conjugate: Unconjugated

Unconjugated

Size/Concentration:

-/ -

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Overview

Tested Applications
Product Information
Publications (6)
Protocols

データシート［PDF］を印刷

MSDS

Tested Applications

Positive WB detected in	mouse brain tissue, human peripheral blood platelets, rat brain tissue, mouse kidney tissue
Positive IHC detected in	mouse lung tissue, human brain tissue, mouse brain tissue, mouse kidney tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:1000-1:8000
Immunohistochemistry (IHC)	IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 3 publications below
WB	See 4 publications below
IHC	See 2 publications below
IF	See 1 publications below

Product Information

25320-1-AP targets EHD3 in WB, IF, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human, rat, mouse, zebrafish
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag18216 Product name: Recombinant human EHD3 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 353-422 aa of BC156996 Sequence: FPNLKRMQDQLQAQDFSKFQPLKSKLLEVVDDMLAHDIAQLMVLVRQEESQRPIQMVKGGAFEGTLHGPF 相同性解析による交差性が予測される生物種
Full Name	EH-domain containing 3
Calculated molecular weight	535 aa, 61 kDa
Observed molecular weight	61 kDa
GenBank accession number	BC156996
Gene Symbol	EHD3
Gene ID (NCBI)	30845
RRID	AB_2880029
Conjugate	Unconjugated
Form
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	Q9NZN3
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Protocols

Product Specific Protocols
IHC protocol for EHD3 antibody 25320-1-AP	Download protocol
WB protocol for EHD3 antibody 25320-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
zebrafish	WB	Nat Cell Biol Early steps in primary cilium assembly require EHD1/EHD3-dependent ciliary vesicle formation. Authors - Quanlong Lu KD Validated View Article
mouse, zebrafish	WB,IF	Nat Cell Biol Early steps in primary cilium assembly require EHD1/EHD3-dependent ciliary vesicle formation. Authors - Quanlong Lu KD Validated View Article
human	WB	EBioMedicine EHD1 impairs decidualization by regulating the Wnt4/β-catenin signaling pathway in recurrent implantation failure. Authors - Quan Zhou View Article
human	WB	EMBO J Insufficiency of ciliary cholesterol in hereditary Zellweger syndrome. Authors - Tatsuo Miyamoto KO Validated View Article
rat	IHC	Int J Mol Sci Proteomic Analysis of Dysfunctional Liver Sinusoidal Endothelial Cells Reveals Substantial Differences in Most Common Experimental Models of Chronic Liver Diseases Authors - Mar Gil View Article
mouse	IHC	Am J Physiol Renal Physiol Unique miRNome and transcriptome profiles underlie microvascular heterogeneity in mouse kidney Authors - Matthijs Luxen View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Validation Data Gallery

WB analysis using 25320-1-AP

WB analysis of human peripheral blood platelets using 25320-1-AP

WB analysis of rat brain using 25320-1-AP

WB analysis of mouse kidney using 25320-1-AP

IHC staining of mouse lung using 25320-1-AP

IHC staining of mouse lung using 25320-1-AP

IHC staining of mouse kidney using 25320-1-AP

IHC staining of mouse kidney using 25320-1-AP

IHC staining of human brain using 25320-1-AP

IHC staining of human brain using 25320-1-AP

IHC staining of mouse brain using 25320-1-AP

IHC staining of mouse brain using 25320-1-AP

EHD3 Polyclonal antibody

EHD3 Polyclonal Antibody for WB, IHC, ELISA

-/ -

Tested Applications

Recommended dilution

Published Applications

Product Information

Protocols

Publications

Early steps in primary cilium assembly require EHD1/EHD3-dependent ciliary vesicle formation.

Early steps in primary cilium assembly require EHD1/EHD3-dependent ciliary vesicle formation.

EHD1 impairs decidualization by regulating the Wnt4/β-catenin signaling pathway in recurrent implantation failure.

Insufficiency of ciliary cholesterol in hereditary Zellweger syndrome.

Proteomic Analysis of Dysfunctional Liver Sinusoidal Endothelial Cells Reveals Substantial Differences in Most Common Experimental Models of Chronic Liver Diseases

Unique miRNome and transcriptome profiles underlie microvascular heterogeneity in mouse kidney