Validation Data Gallery
Tested Applications
| Positive WB detected in | mouse testis tissue, rat testis tissue |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
29383-1-AP targets EHBP1L1 in WB, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag29796 Product name: Recombinant human EHBP1L1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 549-747 aa of NM_001099409 Sequence: AQGAISRGLGGWEAEAGGSGDLETETEVVGLEVLGTQEKEVEGSGFPETRTLEIEILGALEKEAARSRVLESEVAGTAQCEGLETQETEVGVIETPGTETEVLGTQKTEAGGSGVLQTRTTIAETEVLVTQEISGDLGPLKIEDTIQSEMLGTQETEVEASRVPESEAEGTEAKILGTQEITARDSGVREIEAEIAESD 相同性解析による交差性が予測される生物種 |
| Full Name | EH domain binding protein 1-like 1 |
| Calculated molecular weight | 162 kDa |
| Observed molecular weight | 162 kDa |
| GenBank accession number | NM_001099409 |
| Gene Symbol | EHBP1L1 |
| Gene ID (NCBI) | 254102 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q8N3D4 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
EHBP1L1 is an adapter protein localized on recycling endosomes. It regulates apical-directed transport in polarized epithelial cells. Additionally, EHBP1L1 is crucial for erythroblast enucleation by promoting nuclear polarization in coordination with Rab10, Bin1, and dynamin. It also maintains the proper morphology and structural stability of erythrocytes.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for EHBP1L1 antibody 29383-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |