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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using EGR3 Polyclonal antibody (26495-1-AP)

    WB analysis using 26495-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 26495-1-AP (EGR3 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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EGR3 Polyclonal antibody

EGR3 Polyclonal Antibody for WB, ELISA
Cat No. 26495-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Synonyms

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inL02 cells, PC-3 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

26495-1-AP targets EGR3 in WB, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen EGR3 fusion protein Ag24152 相同性解析による交差性が予測される生物種
Full Name early growth response 3
Calculated molecular weight 387 aa, 43 kDa
Observed molecular weight 43 kDa
GenBank accession numberBC104765
Gene Symbol EGR3
Gene ID (NCBI) 1960
RRIDAB_3669540
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ06889
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

The early growth response (Egr) family of zinc finger transcription factors regulates critical genetic programs in cellular growth, differentiation, and function. EGR3 (Early Growth Response 3; also known as PILOT) is a member of the early growth response (Egr) gene family of transcription factors. EGR3 regulates the expression of genes involved in inflammation, including genes encoding various secreted cytokines and protease inhibitors. EGR3 functions as a master regulator of genes differentially expressed in the brains of patients with neuropsychiatric illnesses ranging from schizophrenia and bipolar disorder to Alzheimer's disease.

Protocols

Product Specific Protocols
WB protocol for EGR3 antibody 26495-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols