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- KD/KO Validated
PHD2/EGLN1 Polyclonal antibody
PHD2/EGLN1 Polyclonal Antibody for WB, IP, IHC, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
WB, IP, IHC, ELISA
Conjugate
Unconjugated
Cat no : 19886-1-AP
Synonyms
Validation Data Gallery
Tested Applications
| Positive WB detected in | HCT 116 cells, mouse kidney tissue, rat heart tissue, HEK-293 cells, HEK-293T cells, HepG2 cells |
| Positive IP detected in | HEK-293 cells |
| Positive IHC detected in | human liver cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Sample-dependent, check data in validation data gallery | |
Published Applications
| KD/KO | See 2 publications below |
| WB | See 11 publications below |
| IHC | See 2 publications below |
Product Information
19886-1-AP targets PHD2/EGLN1 in WB, IP, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, rat, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | PHD2/EGLN1 fusion protein Ag13706 相同性解析による交差性が予測される生物種 |
| Full Name | egl nine homolog 1 (C. elegans) |
| Calculated molecular weight | 426 aa, 46 kDa |
| Observed molecular weight | 46 kDa |
| GenBank accession number | BC005369 |
| Gene symbol | EGLN1 |
| Gene ID (NCBI) | 54583 |
| RRID | AB_10641986 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
EGLN1(Egl nine homolog 1) is also named as HIF-PH2,HPH-2,PHD2.It is the most important isozyme under normoxia and, through regulating the stability of HIF1, involved in various hypoxia-influenced processes such as angiogenesis in retinal and cardiac functionality. EGLN1 plays a critical role in regulating HIF levels in EPO-producing cells in humans(PMID:16407130).Defects in EGLN1 are the cause of familial erythrocytosis type 3 (ECYT3).This antibody is specific to EGLN1.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for PHD2/EGLN1 antibody 19886-1-AP | Download protocol |
| IHC protocol for PHD2/EGLN1 antibody 19886-1-AP | Download protocol |
| IP protocol for PHD2/EGLN1 antibody 19886-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Cell Res Hypoxia induces mitochondrial protein lactylation to limit oxidative phosphorylation
| ||
Environ Int HIF-1α/m6A/NF-κB/CCL3 axis-mediated immunosurveillance participates in low level benzene-related erythrohematopoietic development toxicity | ||
Cell Mol Life Sci Iron increases lipid deposition via oxidative stress-mediated mitochondrial dysfunction and the HIF1α-PPARγ pathway. | ||
Int J Mol Sci Sustained Hypoxia Suppresses Joint Destruction in a Rat Model of Rheumatoid Arthritis via Negative Feedback of Hypoxia Inducible Factor-1α. | ||
Biomed Pharmacother A novel PHD2 inhibitor acteoside from Cistanche tubulosa induces skeletal muscle mitophagy to improve cancer-related fatigue. | ||
Sci Rep Mitochondrial MUL1 E3 ubiquitin ligase regulates Hypoxia Inducible Factor (HIF-1α) and metabolic reprogramming by modulating the UBXN7 cofactor protein. |
