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Validation Data Gallery

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  • Western Blot (WB) analysis of A431 cells using EGFR (C-terminal) Polyclonal antibody (51071-2-AP)

    WB analysis of A431 using 51071-2-AP (same clone as 51071-2-PBS)

    A431 cells were subjected to SDS PAGE followed by western blot with 51071-2-AP (EGFR (C-terminal) antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 51071-2-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HeLa cells using EGFR (C-terminal) Polyclonal antibody (51071-2-AP)

    WB analysis of HeLa using 51071-2-AP (same clone as 51071-2-PBS)

    HeLa cells were subjected to SDS PAGE followed by western blot with 51071-2-AP (EGFR (C-terminal) antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 51071-2-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of L02 cells using EGFR (C-terminal) Polyclonal antibody (51071-2-AP)

    WB analysis of L02 using 51071-2-AP (same clone as 51071-2-PBS)

    L02 cells were subjected to SDS PAGE followed by western blot with 51071-2-AP (EGFR (C-terminal) antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 51071-2-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of A431 cells using EGFR (C-terminal) Polyclonal antibody (51071-2-AP)

    IP experiment of A431 using 51071-2-AP (same clone as 51071-2-PBS)

    IP result of anti-EGFR (C-terminal) (IP:51071-2-AP, 4ug; Detection:51071-2-AP 1:30000) with A431 cells lysate 1200 ug. This data was developed using the same antibody clone with 51071-2-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of A431 cells using EGFR (C-terminal) Polyclonal antibody (51071-2-AP)

    IF Staining of A431 using 51071-2-AP (same clone as 51071-2-PBS)

    Immunofluorescent analysis of (4% PFA) fixed A431 cells using EGFR (C-terminal) antibody (51071-2-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 51071-2-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

51071-2-PBS targets EGFR (C-terminal) in WB, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide 相同性解析による交差性が予測される生物種
Full Name epidermal growth factor receptor (erythroblastic leukemia viral (v-erb-b) oncogene homolog, avian)
Calculated molecular weight 1210 aa, 134 kDa
Observed molecular weight 170 kDa
GenBank accession numberBC094761
Gene Symbol EGFR
Gene ID (NCBI) 1956
RRIDAB_2096449
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP00533
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

EGFR, also named as ERBB1, is a cell-surface receptor for members of the epidermal growth factor family (EGF-family) of extracellular protein ligands. Binding of the protein to a ligand induces receptor dimerization and tyrosine autophosphorylation and leads to cell proliferation. The gene resides on chromosome 7p12, encoding a 170 kDa membrane-associated glycoprotein. Recent studies have shown EGFR plays a critical role in cancer development and progression, including cell proliferation, apoptosis, angiogenesis, and metastatic spread. Mutations in this gene are associated with lung cancer. The antibody is specific to isoform1.