Validation Data Gallery
Tested Applications
| Positive WB detected in | human retina tissue, PC-3 cells |
| Positive IF-P detected in | rat eye tissue |
| Positive IF/ICC detected in | Y79 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunofluorescence (IF)-P | IF-P : 1:200-1:800 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| IHC | See 1 publications below |
Product Information
66232-1-Ig targets Pikachurin in WB, IHC, IF/ICC, IF-P, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag6362 Product name: Recombinant human EGFLAM protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 1-152 aa of BC033177 Sequence: MRFKTTAKDGLLLWRGDSPMRPNSDFISLGLRDGALVFSYNLGSGVASIMVNGSFNDGRWHRVKAVRDGQSGKITVDDYGARTGKSPGMMRQLNINGALYVGGMKEIALHTNRQYMRGLVGCISHFTLSTDYHISLVEDSVDGKNINTCGAK 相同性解析による交差性が予測される生物種 |
| Full Name | EGF-like, fibronectin type III and laminin G domains |
| Calculated molecular weight | 111 kDa |
| Observed molecular weight | 111 kDa, 55-60 kDa |
| GenBank accession number | BC033177 |
| Gene Symbol | Pikachurin |
| Gene ID (NCBI) | 133584 |
| RRID | AB_2881623 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Protein G purification |
| UNIPROT ID | Q63HQ2 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Pikachurin, also known as EGFLAM or FLJ39155, is an extracellular matrix-like retinal protein that has been found to localize in the synaptic cleft of the photoreceptor ribbon synapse (PMID: 18641643). Pikachurin is a dystroglycan-interacting protein and has an essential role in photoreceptor ribbon synapse formation. Pikachurin is a 1017-amino-acid protein with a calculated molecular weight of 111 kDa and high mRNA abundance in retina. This monoclonal antibody detects two bands of 111 kDa and 55-60 kDa. The lower-molecular-weight band has been reported and likely represents a fragment resulting from cleavage of Pikachurin (PMID: 23226524).
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for Pikachurin antibody 66232-1-Ig | Download protocol |
| WB protocol for Pikachurin antibody 66232-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |